IGF-II and IL-2 act synergistically to alter HDAC1 expression following treatments with trichostatin A

被引:13
作者
Gray, SG
Svechnikova, I
Hartmann, W
O'Connor, L
Aguilar-Santelises, M
Ekström, TJ
机构
[1] Karolinska Inst, Dept Clin Neurosci, Expt Alcohol & Drug Addict Res Sect, Lab Mol Dev & Tumor Biol, S-17176 Stockholm, Sweden
[2] Womens Hosp, ENH Res Inst, Evanston, IL 60201 USA
关键词
IL-2; IGF-II; Hep3B; trichostatin; histone deacetylase;
D O I
10.1006/cyto.2000.0680
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Histone deacetylases play key roles in the regulation of gene transcription. Studies have shown that expression of interleukins IL-2 and IL-8, and insulin-like growth factor 2 (IGF2) are affected by treatment with histone deacetylase inhibitors. We have previously shown that the gene for histone deacetylase 1 (HDAC1) is upregulated following treatment with TSA, The murine homologue of this gene has been reported to be inducible by IL-2, In this study, we have examined the effects IL-2, IGF-II and TSA have on HDAC1 expression in the human hepatocellular carcinoma derived cell line Hep3B. Our results indicate that in contrast to the mouse, HDAC1 is not inducible by IL-2, However, in TSA treated cells, IL-2 and IC;PII sere found to act synergistically to reduce TSA induced HDAC1 mRNA levels almost to normal. (C) 2000 Academic Press.
引用
收藏
页码:1104 / 1109
页数:6
相关论文
共 15 条
  • [1] AGUILARSANTELISES M, 1994, LEUKEMIA, V8, P1146
  • [2] ACETYLATION + METHYLATION OF HISTONES + THEIR POSSIBLE ROLE IN REGULATION OF RNA SYNTHESIS
    ALLFREY, VG
    FAULKNER, R
    MIRSKY, AE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (05) : 786 - +
  • [3] Identification of mouse histone deacetylase 1 as a growth factor-inducible gene
    Bartl, S
    Taplick, J
    Lagger, G
    Khier, H
    Kuchler, K
    Seiser, C
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (09) : 5033 - 5043
  • [4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
    CHOMCZYNSKI, P
    SACCHI, N
    [J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
  • [5] Gray SG, 1999, INT J MOL MED, V4, P333
  • [6] Effects of cell density and trichostatin A on the expression of HDAC1 and p57Kip2 in Hep 3B cells
    Gray, SG
    Ekström, TJ
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 245 (02) : 423 - 427
  • [7] IGF-II enhances Trichostatin A-induced TGFβ1 and p21Waf1,Cip1,Sdi1 expression in Hep3B cells
    Gray, SG
    Yakovleva, T
    Hartmann, W
    Tally, M
    Bakalkin, G
    Ekström, TJ
    [J]. EXPERIMENTAL CELL RESEARCH, 1999, 253 (02) : 618 - 628
  • [8] The role of histone acetylation in the allelic expression of the imprinted human insulin-like growth factor II gene
    Hu, JF
    Oruganti, H
    Vu, TH
    Hoffman, AR
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 251 (02) : 403 - 408
  • [9] Inhibition of IL-8 gene expression in Caco-2 cells by compounds which induce histone hyperacetylation
    Huang, N
    Katz, JP
    Martin, DR
    Wu, GD
    [J]. CYTOKINE, 1997, 9 (01) : 27 - 36
  • [10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
    LAEMMLI, UK
    [J]. NATURE, 1970, 227 (5259) : 680 - +