IGF-II and IL-2 act synergistically to alter HDAC1 expression following treatments with trichostatin A

Histone deacetylases play key roles in the regulation of gene transcription. Studies have shown that expression of interleukins IL-2 and IL-8, and insulin-like growth factor 2 (IGF2) are affected by treatment with histone deacetylase inhibitors. We have previously shown that the gene for histone deacetylase 1 (HDAC1) is upregulated following treatment with TSA, The murine homologue of this gene has been reported to be inducible by IL-2, In this study, we have examined the effects IL-2, IGF-II and TSA have on HDAC1 expression in the human hepatocellular carcinoma derived cell line Hep3B. Our results indicate that in contrast to the mouse, HDAC1 is not inducible by IL-2, However, in TSA treated cells, IL-2 and IC;PII sere found to act synergistically to reduce TSA induced HDAC1 mRNA levels almost to normal. (C) 2000 Academic Press.