Stimulatory effect of caffeic acid on α1A-adrenoceptors to increase glucose uptake into cultured C2C12 cells

被引:43
作者
Cheng, JT [1 ]
Liu, IM [1 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Pharmacol, Tainan 70101, Taiwan
关键词
C2C12; cells; alpha(1A)-adrenoceptor; phospholipase C; protein kinase C;
D O I
10.1007/s002100000274
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
In an attempt to understand the antihyperglycemic action of caffeic acid, the myoblast C2C12 cells were employed to investigate the glucose uptake in the present study. Caffeic acid enhanced the uptake of radioactive glucose into C2C12 cells in a concentration-dependent manner. Similar effect of phenylephrine on the uptake of radioactive glucose was also observed in C2C12 cells. Prazosin attenuated the action of caffeic acid in a way parallel to the blockade of phenylephrine. Effect of caffeic acid on al-adrenoceptors was further supported by the displacement of [H-3]prazosin binding in C2C12 cells. Moreover, the glucose uptake-increasing action of phenylephrine in C2C12 cells was inhibited by the antagonists of alpha(1A)-adrenoceptors, both tamsulosin and WB 4101, but not by the antagonist of alpha(1B)-adrenoceptors, chlorethylclonidine (CEC). The presence of alpha(1A)-adrenoceptors in C2C12 cells can thus be considered. Similar inhibition of the action of caffeic acid was also obtained in C2C12 cells co-incubating these antagonists. An activation of alpha(1A)-adrenoceptors seems responsible for the action of caffeic acid in C2C12 cells. In the presence of U73312, the specific inhibitor of phospholipase C, caffeic acid-stimulated uptake of radioactive glucose into C2C12 cells was reduced in a concentration-dependent manner and it was not affected by U73343, the negative control of U73312. Moreover, chelerythrine and GF 109203X diminished the action of caffeic acid at concentrations sufficient to inhibit protein kinase C. Therefore, the obtained data suggest that an activation of alpha(1A)-adrenoceptors in C2C12 cells by caffeic acid may increase the glucose uptake via phospholipase C-protein kinase C pathway.
引用
收藏
页码:122 / 127
页数:6
相关论文
共 27 条
[1]  
CAI JF, 1995, ADV TXB TRADITIONAL, V2, P29
[2]   Tissue-specific effects of in vivo adenosine receptor blockade on glucose uptake in Zucker rats [J].
Crist, GH ;
Xu, BY ;
Lanoue, KF ;
Lang, CH .
FASEB JOURNAL, 1998, 12 (13) :1301-1308
[3]   THE TRIUMVIRATE - BETA-CELL, MUSCLE, LIVER - A COLLUSION RESPONSIBLE FOR NIDDM [J].
DEFRONZO, RA .
DIABETES, 1988, 37 (06) :667-687
[4]  
Faintrenie G, 1998, J PHARMACOL EXP THER, V286, P607
[5]   FURTHER EVIDENCE IMPLICATING DIACYLGLYCEROL GENERATION AND PROTEIN-KINASE-C ACTIVATION IN AGONIST-INDUCED INCREASES IN GLUCOSE-UPTAKE - INSULIN-LIKE EFFECTS OF PHENYLEPHRINE IN BC3H-1 MYOCYTES [J].
FARESE, RV ;
ROSIC, N ;
STANDAERT, M ;
BABISCHKIN, J ;
COOPER, DR ;
DAVIS, JS ;
POLLET, RJ .
DIABETES, 1986, 35 (09) :951-957
[6]   ALPHA(1A)-ADRENOCEPTOR-MEDIATED CONTRACTILE RESPONSES OF THE HUMAN VAS-DEFERENS [J].
FURUKAWA, K ;
ROSARIO, DJ ;
SMITH, DJ ;
CHAPPLE, CR ;
UCHIYAMA, T ;
CHESSWILLIAMS, R .
BRITISH JOURNAL OF PHARMACOLOGY, 1995, 116 (01) :1605-1610
[7]   A comparison of the antagonistic activities of tamsulosin and terazosin against human vascular α1-adrenoceptors [J].
Harada, K ;
Ohmori, M ;
Kitoh, Y ;
Sugimoto, K ;
Fujimura, A .
JAPANESE JOURNAL OF PHARMACOLOGY, 1999, 80 (03) :209-215
[8]   CHELERYTHRINE IS A POTENT AND SPECIFIC INHIBITOR OF PROTEIN-KINASE-C [J].
HERBERT, JM ;
AUGEREAU, JM ;
GLEYE, J ;
MAFFRAND, JP .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 172 (03) :993-999
[9]  
HIEBLE JP, 1995, PHARMACOL REV, V47, P267
[10]   Caffeic acid as active principle from the fruit of Xanthium strumarium to lower plasma glucose in diabetic rats [J].
Hsu, FL ;
Chen, YC ;
Cheng, JT .
PLANTA MEDICA, 2000, 66 (03) :228-230