The polymerase chain reaction: from functional genomics to high-school practical classes

被引：10

作者：

Taylor, GR ^{[1
]}

Robinson, P

机构：

[1] St James Univ Hosp, DNA Lab, Leeds LS9 7TF, W Yorkshire, England

[2] St James Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England

[3] St James Univ Hosp, Leeds Dent Inst, Leeds LS9 7TF, W Yorkshire, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1998年 / 9卷 / 01期

关键词：

D O I：

10.1016/S0958-1669(98)80081-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

After a decade of intensive use as an in vitro alternative to cloning DNA, PCR is now well established as the default method for DNA and RNA analysis. Recent developments have consolidated this position by the introduction of more robust formats, improvements in thermal cyclers and labelling and detection methods. The trend is towards increasing automation, although comparatively few diagnostic kits based on PCR are in wide use. At the same time the applications of PCR are being extended with modifications such as long, accurate PCR and arrayed oligonucleotides or expressed sequences. (C) Current Biology Ltd ISSN 0958-1669.

引用

页码：35 / 42

页数：8

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