Investigation of retention on bare silica using reversed-phase mobile phases at elevated temperatures

被引：34

作者：

Bidlingmeyer, BA ^{[1
]}

Henderson, J ^{[1
]}

机构：

[1] Agilent Technol, Wilmington, DE 19808 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1060卷 / 1-2期

关键词：

temperature effects; Van't Hoff plot; silica; retention behavior;

D O I：

10.1016/j.chroma.2004.08.113

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of unbonded silica as a stationary phase in reversed-phase HPLC is described as a useful alternative to bonded phase columns for polar, lipophilic amines. Using four lipophilic amines, the role of temperature is shown to favorably impact both efficiency and selectivity, which is not universally seen when using bonded phases. As temperature is raised, retention drops on the silica column. The temperature behavior appears to support the hypothesis that retention is dependant upon electrostatic and adsorptive forces. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：187 / 193

页数：7

共 36 条

[1] IMPROVEMENTS IN LIQUID-CHROMATOGRAPHY COLUMN LIFE AND METHOD FLEXIBILITY BY SATURATING THE MOBILE PHASE WITH SILICA [J].