Kinetics of engraftment of CD34- and CD34+ cells from mobilized blood differs from that of CD34- and CD34+ cells from bone marrow

被引:51
作者
Verfaillie, CM
Almeida-Porada, G
Wissink, S
Zanjani, ED
机构
[1] Univ Nevada, Vet Affairs Med Ctr, Dept Med, Reno, NV 89502 USA
[2] Univ Minnesota, Sch Med, Dept Med, Stem Cell Biol Program, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Sch Med, Ctr Canc, Minneapolis, MN 55455 USA
关键词
hemopoiesis; Lin(-)CD34(-) cells; long-term engraftment; mobilized blood;
D O I
10.1016/S0301-472X(00)00506-3
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. Mobilized peripheral blood (PB) progenitors are increasingly used in autologous and allogeneic transplantation. However, the short- and long-term engraftment potential of mobilized PB or bone marrow (BM) has not been directly compared. Although several studies showed that BM-derived Lin(-)CD34(-) cells contain hemopoietic progenitors, no studies have addressed whether Lin(-)CD34(-) cells from mobilized PB contain hemopoietic progenitors. Here, we compared the short- and long-term engraftment potential of CD34(+) cells and Lin(-)CD34(-) cells in BM and PB of normal donors who received 5 days of granulocyte colony-stimulating factor (G-CSF), Materials and Methods, 35 x 10(3) CD34(+) or Lin(-)CD34(-) cells from G-CSF mobilized BM and PB of normal donors were transplanted in 60-day-old fetal sheep. Animals were evaluated 2 and 6 months after transplantation for human hemopoietic cells. In addition, cells recovered after 2 months from fetal sheep were serially passaged to secondary and tertiary recipients to assess long-term engrafting cells. Results, Mobilized PB CD34(+) cells supported earlier development of human hemopoiesis than BM CD34(+) cells. When serially transferred to secondary and tertiary recipients, earlier exhaustion of human hematopoiesis was seen for PB than BM CD34(+) cells. A similar degree of chimerism was seen for Lin(-)CD34(-) cells from PB or BM in primary recipients, We again observed earlier exhaustion of human hemopoiesis with serial transplantation of PB than BM Lin(-)CD34(-) cells, Conclusions, Differences exist in the short- and long-term repopulating ability of cells in PB and BM from G-CSF mobilized normal donors, and this is independent of the phenotype, Studies are ongoing to examine if this reflects intrinsic differences in the repopulating potential between progenitors from PB and BM, or a lower frequency of long-term repopulating cells in PB than BM CD34(+) and Lin(-)CD34(-) cells, that may not be apparent if larger numbers of cells are transplanted. (C) 2000 International Society for Experimental Hematology, Published by Elsevier Science Inc.
引用
收藏
页码:1071 / 1079
页数:9
相关论文
共 35 条
[1]   TRANSPLANTATION OF ALLOGENEIC PERIPHERAL-BLOOD STEM-CELLS MOBILIZED BY RECOMBINANT HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR [J].
BENSINGER, WI ;
WEAVER, CH ;
APPELBAUM, FR ;
ROWLEY, S ;
DEMIRER, T ;
SANDERS, J ;
STORB, R ;
BUCKNER, CD .
BLOOD, 1995, 85 (06) :1655-1658
[2]   Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice [J].
Bhatia, M ;
Wang, JCY ;
Kapp, U ;
Bonnet, D ;
Dick, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) :5320-5325
[3]  
Bhatia M, 1997, BLOOD, V90, P1134
[4]   Sustained, retransplantable, multilineage engraftment of highly purified adult human bone marrow stem cells in vivo [J].
Civin, CI ;
AlmeidaPorada, G ;
Lee, MJ ;
Olweus, J ;
Terstappen, LWMM ;
Zanjani, ED .
BLOOD, 1996, 88 (11) :4102-4109
[5]   HUMAN ALLOGENEIC STEM-CELL MAINTENANCE AND DIFFERENTIATION IN A LONG-TERM MULTILINEAGE SCID-HU GRAFT [J].
FRASER, CC ;
KANESHIMA, H ;
HANSTEEN, G ;
KILPATRICK, M ;
HOFFMAN, R ;
CHEN, BP .
BLOOD, 1995, 86 (05) :1680-1693
[6]   Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species [J].
Goodell, MA ;
Rosenzweig, M ;
Kim, H ;
Marks, DF ;
DeMaria, M ;
Paradis, G ;
Grupp, SA ;
Sieff, CA ;
Mulligan, RC ;
Johnson, RP .
NATURE MEDICINE, 1997, 3 (12) :1337-1345
[7]  
Harrison DE, 1997, EXP HEMATOL, V25, P293
[8]   Phenotypic, molecular, and functional characterization of human peripheral blood CD34(+)/Thy1(+) cells [J].
Humeau, L ;
Bardin, F ;
Maroc, C ;
Alario, T ;
Galindo, R ;
Mannoni, P ;
Chabannon, C .
BLOOD, 1996, 87 (03) :949-955
[9]   ALLOGENEIC BLOOD STEM-CELL TRANSPLANTATION FOR REFRACTORY LEUKEMIA AND LYMPHOMA - POTENTIAL ADVANTAGE OF BLOOD OVER MARROW ALLOGRAFTS [J].
KORBLING, M ;
PRZEPIORKA, D ;
HUH, YO ;
ENGEL, H ;
VANBESIEN, K ;
GIRALT, S ;
ANDERSSON, B ;
KLEINE, HD ;
SEONG, D ;
DEISSEROTH, AB ;
ANDREEFF, M ;
CHAMPLIN, R .
BLOOD, 1995, 85 (06) :1659-1665
[10]  
Leung W, 1999, Biol Blood Marrow Transplant, V5, P69, DOI 10.1053/bbmt.1999.v5.pm10371358