Characterization of three DNA transposons in the Dutch elm disease fungi and evidence of repeat-induced point (RIP) mutations

被引:16
作者
Bouvet, Guillaume F. [1 ]
Jacobi, Volker [1 ]
Bernier, Louis [1 ]
机构
[1] Univ Laval, CEF, Quebec City, PQ G1K 7P4, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Ophiostoma novo-ulmi; O; ulmi; DNA transposon; interspecific hybrids; genetic bridge; repeat-induced point mutation (RIP); cumulative transition score (CTS);
D O I
10.1016/j.fgb.2006.11.016
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Transposable elements (TEs) are fundamental components of eukaryotic genomes and can contribute in various ways to genome plasticity and evolution. We describe here the first three DNA transposons in the Dutch elm disease (DED) pathogens Ophiostoma ulmi and O. novo-ulmi, named OPHIO1, OPHIO2 and OPHIO3. We demonstrate that OPHIO transposons, which show high homology to Fot1l pogo TEs within the Tcl/mariner superfamily, have different distribution patterns and specificity in the DED fungi and that interspecific hybrids could act as genetic bridges for transmission of TEs between closely related fungal species. OPHIO3 was found to have undergone repeat-induced point mutations (RIP). We have also developed a complementary method to Margolin's ratios based on the computation of cumulative transition scores (CTS) in order to visualize rapidly RIP signatures on individual DNA strands of OPHIO transposons and TEs found in other ascomycete fungi. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:430 / 443
页数:14
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