Protein analysis on a proteomic scale

被引:458
作者
Phizicky, E
Bastiaens, PIH
Zhu, H
Snyder, M
Fields, S
机构
[1] Univ Rochester, Sch Med, Dept Biochem & Biophys, Rochester, NY 14642 USA
[2] European Mol Biol Lab, D-69117 Heidelberg, Germany
[3] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[4] Univ Washington, Howard Hughes Med Inst, Dept Genome Sci, Seattle, WA 98195 USA
[5] Univ Washington, Dept Med, Seattle, WA 98195 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1038/nature01512
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The long-term challenge of proteomics is enormous: to define the identities, quantities, structures and functions of complete complements of proteins, and to characterize how these properties vary in different cellular contexts. One critical step in tackling this goal is the generation of sets of clones that express a representative of each protein of a proteome in a useful format, followed by the analysis of these sets on a genome-wide basis. Such studies enable genetic, biochemical and cell biological technologies to be applied on a systematic level, leading to the assignment of biochemical activities, the construction of protein arrays, the identification of interactions, and the localization of proteins within cellular compartments.
引用
收藏
页码:208 / 215
页数:8
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