Abattoir sources of psychrophilic clostridia causing blown pack spoilage of vacuum-packed chilled meats determined by culture-based and molecular detection procedures

被引：26

作者：

Boerema, JA ^{[1
]}

Broda, DM ^{[1
]}

Bell, RG ^{[1
]}

机构：

[1] AgRes Ltd, Ruakura MIRINZ Ctr, Hamilton, New Zealand

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2003年 / 36卷 / 06期

关键词：

'blown pack'; clostridia; meat spoilage;

D O I：

10.1046/j.1472-765X.2003.01332.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To identify the abattoir source(s) of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats. Methods and Results: Molecular procedures were used to detect the presence of specific 16S rRNA gene fragments of blown pack-causing clostridia in samples collected from a commercial abattoir and its environs. Blown pack-causing clostridia were consistently detected in hide, soil and faecal samples, as well as in samples collected at slaughter plant locations associated with handling of animals and animal carcasses prior to pelt removal. Conclusions: The data indicate that pelts per se or soil particles/faecal material attached thereto are the most probable primary reservoir of blown pack clostridia in the abattoir. Significance and Impact of the Study: The paper provides information critical for controlling blown pack spoilage in commercial meat-processing plants.

引用

收藏

页码：406 / 411

页数：6

相关论文

共 10 条

[1] Distribution and sources of microbial contamination on beef carcasses [J].

Bell, RG .

JOURNAL OF APPLIED MICROBIOLOGY, 1997, 82 (03) :292-300

[2] Psychrotrophic Clostridium spp associated with 'blown pack' spoilage of chilled vacuum-packed red meats and dog rolls in gas-impermeable plastic casings [J].

Broda, DM ;

DeLacy, KM ;

Bell, RG ;

Braggins, TJ ;

Cook, RL .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 29 (2-3) :335-352

[3] Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotrophic clostridia associated with 'blown pack' spoilage of vacuum-packed meats [J].

Broda, DM ;

Musgrave, DR ;

Bell, RG .

JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (01) :107-116

[4] PCR detection of psychrophilic Clostridium spp. causing 'blown pack' spoilage of vacuum-packed chilled meats [J].

Broda, DM ;

Boerema, JA ;

Bell, RG .

JOURNAL OF APPLIED MICROBIOLOGY, 2003, 94 (03) :515-522

[5] SPOILAGE OF VACUUM-PACKED BEEF BY A CLOSTRIDIUM SP [J].

DAINTY, RH ;

EDWARDS, RA ;

HIBBARD, CM .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1989, 49 (04) :473-486

[6] SPOILAGE OF VACUUM-PACKAGED REFRIGERATED BEEF BY CLOSTRIDIUM [J].

KALCHAYANAND, N ;

RAY, B ;

FIELD, RA ;

JOHNSON, MC .

JOURNAL OF FOOD PROTECTION, 1989, 52 (06) :424-426

[7] THE COMBINED EFFECT OF INCUBATION-TEMPERATURE, PH AND SORBIC ACID ON THE PROBABILITY OF GROWTH OF NONPROTEOLYTIC, TYPE-B CLOSTRIDIUM-BOTULINUM [J].

LUND, BM ;

GRAHAM, AF ;

GEORGE, SM ;

BROWN, D .

JOURNAL OF APPLIED BACTERIOLOGY, 1990, 69 (04) :481-492

[8] THE EXTRACTION AND PURIFICATION OF MICROBIAL DNA FROM SEDIMENTS [J].

OGRAM, A ;

SAYLER, GS ;

BARKAY, T .

JOURNAL OF MICROBIOLOGICAL METHODS, 1987, 7 (2-3) :57-66

[9] Detection of pathogenic and spoilage micro-organisms in food with the polymerase chain reaction [J].

Scheu, PM ;

Berghof, K ;

Stahl, U .

FOOD MICROBIOLOGY, 1998, 15 (01) :13-31

[10] RAPID DNA EXTRACTION PROTOCOL FROM SOIL FOR POLYMERASE CHAIN REACTION-MEDIATED AMPLIFICATION [J].

SMALLA, K ;

CRESSWELL, N ;

MENDONCAHAGLER, LC ;

WOLTERS, A ;

VANELSAS, JD .

JOURNAL OF APPLIED BACTERIOLOGY, 1993, 74 (01) :78-85