Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins

被引：67

作者：

Takahashi, Y ^{[1
]}

Mizoi, J ^{[1
]}

Toh-e, A ^{[1
]}

Kikuchi, Y ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2000年 / 128卷 / 05期

关键词：

hydrolase; nucleoporins; Smt3; SUMO-1; Ulp1;

D O I：

10.1093/oxfordjournals.jbchem.a022807

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast Smt3 is a ubiquitin-like protein similar to the mammalian SUMO-1. Cdc3, a septin component, is known to be modified by Smt3, The level of this modification was affected by Smt3-specific protease mutation ulp1-ts or overexpression of ULP1. By two-hybrid screening, we isolated 5 UIP (Ulp1 Interacting protein) genes. UIP1 was identical to NUP42 encoding a component of the nuclear pore complex (NPC), Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and coimmunoprecipitation experiment. Thus Ulp1 associates with nucleoporins and may interact with septin rings in the telophase.

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页码：723 / 725

页数：3

相关论文

共 17 条

[1] AN ESSENTIAL YEAST GENE ENCODING A HOMOLOG OF UBIQUITIN-ACTIVATING ENZYME [J].

DOHMEN, RJ ;

STAPPEN, R ;

MCGRATH, JP ;

FORROVA, H ;

KOLAROV, J ;

GOFFEAU, A ;

VARSHAVSKY, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) :18099-18109

[2]

James P, 1996, GENETICS, V144, P1425

[3] Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins [J].

Johnson, ES ;

Blobel, G .

JOURNAL OF CELL BIOLOGY, 1999, 147 (05) :981-993

[4] Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p [J].

Johnson, ES ;

Blobel, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) :26799-26802

[5] The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer [J].

Johnson, ES ;

Schwienhorst, I ;

Dohmen, RJ ;

Blobel, G .

EMBO JOURNAL, 1997, 16 (18) :5509-5519

[6] The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein [J].

Li, SJ ;

Hochstrasser, M .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (07) :2367-2377

[7] A new protease required for cell-cycle progression in yeast [J].

Li, SJ ;

Hochstrasser, M .

NATURE, 1999, 398 (6724) :246-251

[8] Reaction of glyoxal with aliphatic alcohols using cationic exchange resins as catalysts [J].

Mahajani, SM ;

Sharma, MM .

ORGANIC PROCESS RESEARCH & DEVELOPMENT, 1997, 1 (02) :97-105

[9] A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex [J].

Matunis, MJ ;

Coutavas, E ;

Blobel, G .

JOURNAL OF CELL BIOLOGY, 1996, 135 (06) :1457-1470

[10] EVIDENCE THAT THE MIF2 GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A CENTROMERE PROTEIN WITH HOMOLOGY TO THE MAMMALIAN CENTROMERE PROTEIN CENP-C [J].

MELUH, PB ;

KOSHLAND, D .

MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (07) :793-807