Synthesis of oligonucleotides with a 2′-cap at the 3′-terminus via reversed phosphoramidites

被引：18

作者：

Connors, WH

Narayanan, S

Kryatova, OP

Richert, C ^{[1
]}

机构：

[1] Univ Karlsruhe, Inst Organ Chem, Dept Chem, D-76131 Karlsruhe, Germany

[2] Univ Constance, Dept Chem, D-78457 Constance, Germany

[3] Tufts Univ, Dept Chem, Medford, MA 02155 USA

来源：

ORGANIC LETTERS | 2003年 / 5卷 / 03期

关键词：

D O I：

10.1021/ol020212w

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] A method is presented for the synthesis of single compounds or small combinatorial libraries of oligonucleotides with 2'-acylamido-2'-deoxyuridine residues at the 3'-terminus. Selection experiments identified the residue of anthraquinone-2-carboxylic acid as a "molecular cap" that increases the UV melting point of the duplex (5'-ACGCGU-3')(2) by up to 28degreesC compared to the unmodified control duplex.

引用

页码：247 / 250

页数：4

共 35 条

[1] Selection of modified oligonucleotides with increased target affinity via MALDI-monitored nuclease survival assays [J].