DNA-triplex stabilizing properties of 8-aminoguanine

被引：35

作者：

Soliva, R

García, RG

Blas, JR

Eritja, R

Asensio, JL

González, C

Luque, FJ

Orozco, M

机构：

[1] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

[2] European Mol Biol Lab, D-69117 Heidelberg, Germany

[3] CSIC, Inst Estructura Mat, E-28006 Madrid, Spain

[4] CSIC, Inst Quim Organ, E-28006 Madrid, Spain

[5] Univ Barcelona, Fac Farm, Dept Fisicoquim, E-08028 Barcelona, Spain

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 22期

关键词：

D O I：

10.1093/nar/28.22.4531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A DNA-triplex stabilizing purine (8-aminoguanine) is designed based on molecular modeling and synthesized, The substitution of guanine by 8-aminoguanine largely stabilizes the tripler both at neutral and acidic pH, as suggested by molecular dynamics and thermodynamic integration calculations, and demonstrated by melting experiments, NMR experiments confirm the tripler-stabilizing properties of 8-aminoguanine and demonstrate that few changes are found in the structure of the tripler due to the presence of this modified base.

引用

页码：4531 / 4539

页数：9

共 58 条

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