Complexation of antibacterial quinolonic acid and cinolonic derivatives with Zn(II) and Al(III):: application to their determination in human urine

Nalidixic acid, 7-hydroxymethylnalidixic acid, oxolinic acid, pipemidic acid and cinoxacine form complexes with zinc(II) in the presence of acetate buffer of pH 5.5 and oxolinic acid, pipemidic acid and cinoxacine form complexes with aluminium(III) in the presence of chloroacetate buffer of pH 3.0. In all cases, an enhancement of the fluorescence emission was observed. Fluorimetric studies on the spectral characteristics of the complexes were performed. A 1 : 1 stoichiometry for all the complexes was established. The association constants were calculated, by using the changes in the fluorescence of all antibacterials, that occurred when the complexes were formed. The fluorescence reactions were used to develop methods for the determination of all of the above compounds, showing a higher sensitivity than in the absence of the cationic ions. The methods were satisfactorily applied to the determination of these compounds in urine.