Nano Aptasensor for Protective Antigen Toxin of Anthrax

被引：82

作者：

Cella, Lakshmi N. ^{[1
,2
,4
]}

Sanchez, Pablo ^{[1
,3
,4
]}

Zhong, Wenwan ^{[5
]}

Myung, Nosang V. ^{[1
,4
]}

Chen, Wilfred ^{[1
,4
]}

Mulchandani, Ashok ^{[1
,4
]}

机构：

[1] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Cell Mol & Dev Biol Grad Program, Riverside, CA 92521 USA

[3] Univ Calif Riverside, Microbiol Grad Program, Riverside, CA 92521 USA

[4] Univ Calif Riverside, Ctr Nanoscale Sci & Engn, Riverside, CA 92521 USA

[5] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 05期

关键词：

WALLED CARBON NANOTUBES; IN-VITRO SELECTION; BACILLUS-ANTHRACIS; CAPILLARY-ELECTROPHORESIS; ELECTROCHEMICAL DETECTION; LETHAL FACTOR; APTAMERS; AFFINITY; BIOSENSORS; LIGANDS;

D O I：

10.1021/ac902791q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We demonstrate a highly sensitive nano aptasensor for anthrax toxin through the detection of its polypeptide entity, protective antigen (PA toxin) using a PA toxin ssDNA aptamer functionalized single-walled carbon nanotubes (SWNTs) device. The aptamer was developed in-house by capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) and had a dissociation constant (k(d)) of 112 nM. The aptasensor displayed a wide dynamic range spanning up to 800 nM with a detection limit of 1 nM. The sensitivity was 0.11 per nM, and it was reusable six times. The aptasensor was also highly selective for PA toxin with no interference from human and bovine serum albumin, demonstrating it as a potential tool for rapid and point-of-care diagnosis for anthrax.

引用

页码：2042 / 2047

页数：6

共 36 条

[1] Europium-Based Fluorescence Nanoparticle Sensor for Rapid and Ultrasensitive Detection of an Anthrax Biomarker
Ai, Kelong
Zhang, Baohua
Lu, Lehui
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (02) : 304 - 308
[2] Baker BR, 2006, J AM CHEM SOC, V128, P3138, DOI 10.1021/ja056957p
[3] A novel electrochemical detection method for aptamer biosensors
Bang, GS
Cho, S
Kim, BG
[J]. BIOSENSORS & BIOELECTRONICS, 2005, 21 (06) : 863 - 870
[4] Identification of the cellular receptor for anthrax toxin
Bradley, KA
Mogridge, J
Mourez, M
Collier, RJ
Young, JAT
[J]. NATURE, 2001, 414 (6860) : 225 - 229
[5] Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors
Chen, RJ
Bangsaruntip, S
Drouvalakis, KA
Kam, NWS
Shim, M
Li, YM
Kim, W
Utz, PJ
Dai, HJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (09) : 4984 - 4989
[6] Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization
Chen, RJ
Zhang, YG
Wang, DW
Dai, HJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (16) : 3838 - 3839
[7] Pore structure of raw and purified HiPco single-walled carbon nanotubes
Cinke, M
Li, J
Chen, B
Cassell, A
Delzeit, L
Han, J
Meyyappan, M
[J]. CHEMICAL PHYSICS LETTERS, 2002, 365 (1-2) : 69 - 74
[8] CLOAD S, 2002, DAAD1902C0051
[9] The detection of protective antigen (PA) associated with spores of Bacillus anthracis and the effects of anti-PA antibodies on spore germination and macrophage interactions
Cote, CK
Rossi, CA
Kang, AS
Morrow, PR
Lee, JS
Welkos, SL
[J]. MICROBIAL PATHOGENESIS, 2005, 38 (5-6) : 209 - 225
[10] Mapping the lethal factor and edema factor binding sites on oligomeric anthrax protective antigen
Cunningham, K
Lacy, DB
Mogridge, J
Collier, RJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (10) : 7049 - 7053

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