In vivo proton HR-MAS NMR metabolic profile of the freshwater cladoceran Daphnia magna

被引：38

作者：

Bunescu, Andrei ^{[1
,2
]}

Garric, Jeanne ^{[1
]}

Vollat, Bernard ^{[1
]}

Canet-Soulas, Emmanuelle ^{[3
]}

Graveron-Demilly, Danielle ^{[3
]}

Fauvelle, Florence ^{[2
]}

机构：

[1] Cemagref, UR MALY, Lab Ecotoxicol, Lyon, France

[2] CRSSA, NMR Lab, F-38702 La Tronche, France

[3] Univ Lyon 1, CNRS, Lab CREATIS LRMN, UMR 5220,Inserm,U630,INSA Lyon, F-69622 Villeurbanne, France

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 01期

关键词：

FATTY-ACIDS; REPRODUCTION; PHOSPHORUS; EXPOSURE; STRESS; GROWTH;

D O I：

10.1039/b915417e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The method concerning in vivo proton HR-MAS NMR metabolic profiling of the freshwater cladoceran Daphnia magna is presented. Viability tests of D. magna under different spinning rates were performed. All surviving daphnids after analysis have developed eggs and embryos like control animals. Better survival rate at the slowest rotation speed were observed. The maximum length of analysis during which the integrity of the daphnid is maintained was assessed. The recorded proton spectra of in vivo daphnia were attributed to lipids from the triglycerol category. Saturated and unsaturated omega-3 like fatty acid moieties of triacylglycerol were well identified. The relationship between physiological state of daphnids and lipid profile are discussed.

引用

页码：121 / 125

页数：5

共 22 条

[1] Changes in antioxidant enzyme activities, fatty acid composition and lipid peroxidation in Daphnia magna during the aging process [J].

Barata, C ;

Navarro, JC ;

Varo, I ;

Riva, MC ;

Arun, S ;

Porte, C .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2005, 140 (01) :81-90

[2] Differential effects of phosphorus and fatty acids on Daphnia magna growth and reproduction [J].

Becker, C ;

Boersma, M .

LIMNOLOGY AND OCEANOGRAPHY, 2005, 50 (01) :388-397

[3] An improved NMR study of liposomes using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine as model [J].

Cruciani, Oscar ;

Mannina, Luisa ;

Sobolev, Anatoli P. ;

Cametti, Cesare ;

Segre, AnnaLaura .

MOLECULES, 2006, 11 (05) :334-344

[4]

De Coen WM, 1998, HYDROBIOLOGIA, V367, P199, DOI 10.1023/A:1003240502946

[5] HR-MAS NMR analysis of biological tissues: a critical report [J].

Desmoulin, Franck ;

Bon, Delphine ;

Martino, Robert ;

Malet-Martino, Myriam .

COMPTES RENDUS CHIMIE, 2008, 11 (4-5) :423-433

[6] Daphnia need to be gut-cleared too:: the effect of exposure to and ingestion of metal-contaminated sediment on the gut-clearance patterns of D-magna [J].

Gillis, PL ;

Chow-Fraser, P ;

Ranville, JF ;

Ross, PE ;

Wood, CM .

AQUATIC TOXICOLOGY, 2005, 71 (02) :143-154

[7]

Griffin Julian L, 2004, Drug Discov Today Technol, V1, P285, DOI 10.1016/j.ddtec.2004.10.011

[8] NMR for microbiology: In vivo and in situ applications [J].

Grivet, Jean-Philippe ;

Delort, Anne-Marie .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2009, 54 (01) :1-53

[9] Systems biology meets stress ecology:: linking molecular and organismal stress responses in Daphnia magna [J].

Heckmann, Lars-Henrik ;

Sibly, Richard M. ;

Connon, Richard ;

Hooper, Helen L. ;

Hutchinson, Thomas H. ;

Maund, Steve J. ;

Hill, Christopher J. ;

Bouetard, Anthony ;

Callaghan, Amanda .

GENOME BIOLOGY, 2008, 9 (02)

[10]

*ISO, 1989, 634119892 ISO

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