Direct determination of zolpidem and its main metabolites in urine using capillary electrophoresis with laser-induced fluorescence detection

被引：55

作者：

Hempel, G ^{[1
]}

Blaschke, G ^{[1
]}

机构：

[1] UNIV MUNSTER,INST PHARMACEUT CHEM,D-48149 MUNSTER,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 675卷 / 01期

关键词：

zolpidem;

D O I：

10.1016/0378-4347(95)00342-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Zolpidem is a new sleep inducer belonging to the imidazopyridine class. We wish to report a method for the determination of zolpidem and its main metabolites in urine without extraction using capillary electrophoresis with UV laser-induced fluorescence detection with a He-Cd laser. A 10-nl sample of urine can be directly applied to the capillary. The separation is carried out within 10 min, and the limit of detection is 2 ng/ml. This procedure is very simple and fast. No organic solvents are necessary.

引用

页码：131 / 137

页数：7

共 6 条

[1] DETERMINATION OF ZOLPIDEM, A NEW SLEEP-INDUCING AGENT, AND ITS METABOLITES IN BIOLOGICAL-FLUIDS - PHARMACOKINETICS, DRUG-METABOLISM AND OVERDOSING INVESTIGATIONS IN HUMANS [J].

ASCALONE, V ;

FLAMINIO, L ;

GUINEBAULT, P ;

THENOT, JP ;

MORSELLI, PL .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 581 (02) :237-250

[2]

GUZMAN AN, 1993, CAPILLARY ELECTROPHO

[3]

LANGER SZ, 1988, IMIDAZOPYRIDINES SLE, P139

[4] ZOLPIDEM - A REVIEW OF ITS PHARMACODYNAMIC AND PHARMACOKINETIC PROPERTIES AND THERAPEUTIC POTENTIAL [J].

LANGTRY, HD ;

BENFIELD, P .

DRUGS, 1990, 40 (02) :291-313

[5]

SHAH VP, 1991, EUR J DRUG METAB PH, V16, P249

[6]

SMITH NW, 1994, J PHARM BIOMED ANAL, V12, P753

← 1 →