Rabies as a transneuronal tracer of circuits in the central nervous system

被引:234
作者
Kelly, RM
Strick, PL [1 ]
机构
[1] SUNY Upstate Med Univ, Dept Neurosci & Physiol, Syracuse, NY 13210 USA
[2] Univ Pittsburgh, Sch Med, Vet Affairs Med Ctr, Res Serv, Pittsburgh, PA 15261 USA
[3] Univ Pittsburgh, Sch Med, Ctr Neural Basis Cognit, Dept Neurobiol, Pittsburgh, PA 15261 USA
[4] Univ Pittsburgh, Sch Med, Ctr Neural Basis Cognit, Dept Psychiat, Pittsburgh, PA 15261 USA
关键词
central nervous system; rabies; transneuronal tracer; neuroanatomical techniques; virus transport; cerebral cortex; cerebellum; basal ganglia;
D O I
10.1016/S0165-0270(00)00296-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The ability of selected neurotropic viruses to move transneuronally in the central nervous system makes them particularly well suited for use as tracers in experimental neuroanatomy. Recently, techniques have been developed for using rabies virus as a transneuronal tracer. Several features of rabies infection make the virus particularly useful for this purpose. We examined transneuronal transport of rabies in the central nervous system of primates after intracortical and intramuscular injections. Rabies was transported in a time-dependent manner to infect synaptically-connected chains of neurons. Transport occurred exclusively in the retrograde direction. At the survival times we used, rabies infection was restricted to neurons and did not cause cell lysis. There are several methodological and safety issues that must be considered when designing studies that use rabies as a transneuronal tracer. When appropriate protocols and laboratory practices have been established, transneuronal transport of rabies can be a safe and efficient tool for revealing the organization of multi-synaptic circuits in the central nervous system. (C) 2000 Published by Elsevier Science B.V.
引用
收藏
页码:63 / 71
页数:9
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