β2 subunit propeptides influence cooperative proteasome assembly

被引:91
作者
De, M
Jayarapu, K
Elenich, L
Monaco, JJ
Colbert, RA
Griffin, TA [1 ]
机构
[1] Cincinnati Childrens Hosp, Med Ctr, William S Rowe Div Rheumatol, Cincinnati, OH 45229 USA
[2] Univ Cincinnati, Coll Med, Dept Mol Genet, Cincinnati, OH 45267 USA
关键词
D O I
10.1074/jbc.M209292200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Vertebrate proteasomes are structurally heterogeneous, consisting of both "constitutive" (or "standard") proteasomes and "immunoproteasomes." Constitutive proteasomes contain three ubiquitously expressed catalytic subunits, Delta (beta1), Z (beta2), and X (beta5), whereas immunoproteasomes contain three interferon-gamma-inducible catalytic subunits, LMP2 (beta1i), MECL (beta2i), and LMP7 (beta5i). We recently have demonstrated that proteasome assembly is biased to promote immunoproteasome homogeneity when both types of catalytic subunits are expressed in the same cell. This cooperative assembly is due in part to differences between the LMP7 (beta5i) and X (beta5) propeptides. In the current study we demonstrate that differences between the MECL (beta2i) and Z (beta2) propeptides also influence cooperative assembly. Specifically, replacing the MECL propeptide with that of Z enables MECL incorporation into otherwise constitutive (Delta(+)/X+) proteasomes and facilitates X incorporation into otherwise immunoproteasomes (MECL+/LMP2(+)). We also show, using MECL-/- mice, that LMP2 incorporation does not require MECL, in contrast with previous suggestions that their incorporation is mutually codependent. These results enable us to refine our model for cooperative proteasome assembly by determining which combinations of inducible and constitutive subunits are favored over others, and we propose a mechanism for how propeptides mediate cooperative assembly.
引用
收藏
页码:6153 / 6159
页数:7
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