Potential of DNA microarrays for developing parallel detection tools (PDTs) for microorganisms relevant to biodefense and related research needs

被引:30
作者
Hashsham, SA
Wick, LM
Rouillard, JM
Gulari, E
Tiedje, JM
机构
[1] Michigan State Univ, Dept Civil & Environm Engn, E Lansing, MI 48824 USA
[2] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA
[3] Michigan State Univ, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA
[4] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA
[5] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
关键词
DNA microarrays; parallel detection tools; biodefense;
D O I
10.1016/j.bios.2004.06.032
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Development of parallel detection tools using microarrays is critically reviewed in view of the need for screening multiple microorganisms in a single test. Potential research needs with respect to probe design and specificity, validation, sample concentration, selective target enrichment and amplification, and data analysis are discussed. Data illustrating selected probe design issues for detecting multiple targets in mixed microbial systems is presented. Challenges with respect to cost, time, and ease of use compared to other methods are also summarized. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:668 / 683
页数:16
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