Expression and activity of serum response factor is required for expression of the muscle-determining factor MyoD in both dividing and differentiating mouse C2C12 myoblasts

被引:67
作者
GauthierRouviere, C
Vandromme, M
Tuil, D
Lautredou, N
Morris, M
Soulez, M
Kahn, A
Fernandez, A
Lamb, N
机构
[1] CNRS,INSERM,CTR RECH MACROMOL,CELL BIOL UNIT,F-34033 MONTPELLIER,FRANCE
[2] INSERM,INST COCHIN GENET MOLEC,U129,F-75014 PARIS,FRANCE
关键词
D O I
10.1091/mbc.7.5.719
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To understand the mechanism by which the serum response factor (SRF) is involved in the process of skeletal muscle differentiation, we have assessed the effect of inhibiting SRF activity or synthesis on the expression of the muscle-determining factor MyoD. Inhibition of SRF activity in mouse myogenic C2C12 cells through microinjection of either the SRE oligonucleotide (which acts by displacing SRF proteins from the endogenous SRE sequences), purified SRF-DB (a 30-kDa portion of SRF containing the DNA-binding domain of SRF, which acts as a dominant negative mutant in vivo), or purified anti-SRF antibodies rapidly prevents the expression of MyoD. Moreover, the rapid shutdown of MyoD expression after in vivo inhibition of SRF activity is observed not only in proliferating myoblasts but also in myoblasts cultured under differentiating conditions. Additionally, by using a cellular system expressing a glucocorticoid-inducible antisense-SRF (from aa 74 to 244) we have shown that blocking SRF expression by dexamethasone induction of antisense SRF results in the lack of MyoD expression as probed by both immunofluorescence and Northern blot analysis. Taken together these data demonstrate that SRF expression and activity are required for the expression of the muscle-determining factor MyoD.
引用
收藏
页码:719 / 729
页数:11
相关论文
共 49 条
  • [1] CYTOPLASMIC ACTIVATION OF HUMAN NUCLEAR GENES IN STABLE HETEROCARYONS
    BLAU, HM
    CHIU, CP
    WEBSTER, C
    [J]. CELL, 1983, 32 (04) : 1171 - 1180
  • [2] BOXER LM, 1988, J BIOL CHEM, V264, P1284
  • [3] BREITBART RE, 1993, DEVELOPMENT, V118, P1095
  • [4] CATALA F, 1995, MOL CELL BIOL, V15, P4585
  • [5] MYOD CONVERTS PRIMARY DERMAL FIBROBLASTS, CHONDROBLASTS, SMOOTH-MUSCLE, AND RETINAL PIGMENTED EPITHELIAL-CELLS INTO STRIATED MONONUCLEATED MYOBLASTS AND MULTINUCLEATED MYOTUBES
    CHOI, J
    COSTA, ML
    MERMELSTEIN, CS
    CHAGAS, C
    HOLTZER, S
    HOLTZER, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) : 7988 - 7992
  • [6] AN OPPORTUNISTIC PROMOTER SHARING REGULATORY SEQUENCES WITH EITHER A MUSCLE-SPECIFIC OR A UBIQUITOUS PROMOTER IN THE HUMAN ALDOLASE-A GENE
    CONCORDET, JP
    SALMINEN, M
    DEMIGNON, J
    MOCH, C
    MAIRE, P
    KAHN, A
    DAEGELEN, D
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (01) : 9 - 17
  • [7] MYOGENIN INDUCES THE MYOCYTE-SPECIFIC ENHANCER BINDING-FACTOR MEF-2 INDEPENDENTLY OF OTHER MUSCLE-SPECIFIC GENE-PRODUCTS
    CSERJESI, P
    OLSON, EN
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (10) : 4854 - 4862
  • [8] ERNT H, 1991, MOL CEL BIOL, V11, P3735
  • [9] P67SRF IS A CONSTITUTIVE NUCLEAR-PROTEIN IMPLICATED IN THE MODULATION OF GENES REQUIRED THROUGHOUT THE G1 PERIOD
    GAUTHIERROUVIERE, C
    CAVADORE, JC
    BLANCHARD, JM
    LAMB, NJC
    FERNANDEZ, A
    [J]. CELL REGULATION, 1991, 2 (07): : 575 - 588
  • [10] CASEIN KINASE-II INDUCES C-FOS EXPRESSION VIA THE SERUM RESPONSE ELEMENT PATHWAY AND P67SRF PHOSPHORYLATION IN LIVING FIBROBLASTS
    GAUTHIERROUVIERE, C
    BASSET, M
    BLANCHARD, JM
    CAVADORE, JC
    FERNANDEZ, A
    LAMB, NJC
    [J]. EMBO JOURNAL, 1991, 10 (10) : 2921 - 2930