Evaluation of antimicrobial agents for veterinary use in the ecotoxicity test using microalgae

被引:312
作者
Eguchi, K
Nagase, H
Ozawa, M
Endoh, YS
Goto, K
Hirata, K
Miyamoto, K
Yoshimura, H
机构
[1] Minist Agr, Natl Vet Assay Lab, Kokubunji, Tokyo 1858511, Japan
[2] Osaka Univ, Grad Sch Pharmaceut Sci, Environm Biotechnol Lab, Suita, Osaka 5650871, Japan
关键词
algal toxicity; combined drug; synergistic effect; folate;
D O I
10.1016/j.chemosphere.2004.07.017
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The influence of antimicrobial agents approved as veterinary drugs in Japan on the growth of green algae, Selenastrum capricornutum and Chlorella vulgaris, was studied in accordance with the OECD guidelines for testing chemicals. Among the agents tested, growth inhibitory activity was very varied, i.e. erythromycin showed the strongest activity (EC50 50% effective concentration, =0.037 mg/l), sulfa drugs had activity to some extent (EC(50)s of sulfamethoxazole, sulfadiazine, and sulfadimethoxine were 1.5, 2.2, and 2.3 mg/l, respectively), but arnpicillin and cefazolin did not inhibit growth (EC(50)s > 1000mg/1). We also investigated synergistic effect of combining sulfa drugs with trimethoprim or pyrimethamine, which are cormnonly used as a combined drug. By adding trimethoprim, the growth inhibitory activity of sulfamethoxazole and sulfadiazine was significantly enhanced. Growth inhibition by sulfa drugs was reduced by the addition of folic acid, indicating that they inhibit folate synthesis in green algae. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1733 / 1738
页数:6
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