Reference gene selection for real-time quantitative polymerase chain reaction normalization in "Swingle" citrumelo under drought stress

被引：44

作者：

Carvalho, K. ^{[1
]}

de Campos, M. K. F. ^{[1
]}

Pereira, L. F. P. ^{[2
]}

Vieira, L. G. E. ^{[1
]}

机构：

[1] Inst Agron Parana, Lab Biotecnol Vegetal, BR-86047902 Londrina, PR, Brazil

[2] Embrapa Cafe, BR-86047902 Londrina, PR, Brazil

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 402卷 / 02期

关键词：

RT-PCR; EXPRESSION; IDENTIFICATION;

D O I：

10.1016/j.ab.2010.03.038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the first systematic evaluation of reference genes for use in real-time quantitative polymerase chain reaction (qPCR) for water deficit stress studies in the citrus rootstock "Swingle" citrumelo. The expression levels of seven reference genes cyclophilin (CYP), cathepsin (CtP), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1 alpha), beta-tubulin (TUB), and ADP ribosylation factor (ADP) during drought stress were tested using geNorm and NormFinder programs. Results from four experimental conditions indicated that EF1 alpha and ADP were the most stable reference genes. Relative expression levels of Delta 1-pyrroline-5-carboxylate synthetase (P5CS) was used for reference gene validation. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：197 / 199

页数：3

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