Fourier-transform infrared spectroscopy of human cancerous and normal intestine

被引：10

作者：

Mordechai, S ^{[1
]}

Salman, A ^{[1
]}

Argov, S ^{[1
]}

Cohen, B ^{[1
]}

Erukhimovitch, V ^{[1
]}

Goldstein, J ^{[1
]}

Chaims, O ^{[1
]}

Hammody, Z ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel

来源：

BIOMEDICAL SPECTROSCOPY: VIBRATIONAL SPECTROSCOPY AND OTHER NOVEL TECHNIQUES | 2000年 / 3918卷

关键词：

cancer diagnosis; FTIR microscopy; intestinal cancer; epithelial cells; bands fitting;

D O I：

10.1117/12.384956

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Fourier-Transform Infrared Spectroscopy (FTIR) employs a unique approach to optical diagnosis of tissue pathology based on the characteristic molecular vibrational spectra of the tissue. The architectural changes in the cellular and sub-cellular levels developing in abnormal tissue, including a majority of cancer forms, manifest themselves in different optical signatures, which can be detected in infrared spectroscopy. The molecular vibrational modes, which are responsible for infrared (IR) absorption spectra, are characteristic of the biochemistry of the cells and their sub-cellular components. The biological systems we have studied include adenocarcinoma and normal colonic tissues obtained from the department of pathology at Soroka Medical Center (SMC). Our method is based on microscopic infrared study (FTIR-microscopy) of thin tissue specimens and a direct comparison with normal histopathological analysis, which serves as a "gold" reference. Several unique differences between normal and cancerous intestinal specimens have been observed. The cancerous intestine has weaker absorption strength over a wide region, which includes several significant vibrational bands. The results from microscopic IR absorption spectra from intestinal tissues (normal and cancerous) have also been compared with other biological tissue samples.

引用

页码：66 / 77

页数：12

共 26 条

[1] Minimally invasive and ex vivo diagnostics of breast cancer tissues by fiber optic evanescent wave Fourier transform IR (FEW-FTIR) spectroscopy [J].