Mupirocin prophylaxis against methicillin-susceptible, methicillin-resistant, or vancomycin-intermediate Staphylococcus epidermidis vascular-graft infection

被引:10
作者
Giacometti, A
Cirioni, O
Ghiselli, R
Goffi, L
Viticchi, C
Mocchegiani, F
Riva, A
Orlando, F
Saba, V
Scalise, G
机构
[1] Univ Ancona, Inst Infect Dis & Publ Hlth, Ancona, Italy
[2] Univ Ancona, Dept Gen Surg, INRCAIRRCS, Ancona, Italy
[3] INRCA IRRCS, Res Dept, Ctr Biotechnol, Ancona, Italy
关键词
D O I
10.1128/AAC.44.10.2842-2844.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A rat model was used to investigate the efficacy of mupirocin in the prevention of vascular prosthetic graft infection due to Staphylococcus epidermidis strains with different susceptibility patterns (methicillin susceptible, methicillin resistant, and with intermediate resistance to vancomycin). The effect of mupirocin-soaked Dacron was compared to that of perioperative intraperitoneal prophylaxis with vancomycin. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses (1 cm(2)) followed by topical inoculation with 5 x 10(7) CFU of one staphylococcal strain, The study included a control group (no graft contamination), three contaminated groups that did not receive any antibiotic prophylaxis, three contaminated groups that received mupirocin-soaked grafts, three contaminated groups in which perioperative intraperitoneal vancomycin prophylaxis (10 mg/kg of body weight) was administered, and three contaminated groups that received mupirocin-soaked grafts and perioperative intraperitoneal vancomycin prophylaxis (10 mg/kg), The grafts were sterilely removed 7 days after implantation, and the infection,vas evaluated by using sonication and quantitative agar culture, Data analysis showed the efficacy of mupirocin against all three strains, with growth of the strains in treated rats significantly different than that in the untreated control, In addition, mupirocin was more effective than vancomycin against the strain with intermediate susceptibility to the glycopeptide. Finally, the combination of mupirocin and vancomycin produced complete suppression of the growth of all of the strains.
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页码:2842 / 2844
页数:3
相关论文
共 24 条
[1]   TREATMENT OF BACTERIA-BIOFILM GRAFT INFECTION BY IN-SITU REPLACEMENT IN NORMAL AND IMMUNE-DEFICIENT STATES [J].
BANDYK, DF ;
KINNEY, EV ;
RIEFSNYDER, TI ;
KELLY, H ;
TOWNE, JB ;
SMITH, RB ;
BERGAMINI, T ;
ROBICSEK, F ;
BUNT, TJ .
JOURNAL OF VASCULAR SURGERY, 1993, 18 (03) :398-406
[2]  
BERGAMINI TM, 1994, J SURG RES, V56, P393
[3]   PROPHYLACTIC ANTIBIOTICS PREVENT BACTERIAL BIOFILM GRAFT INFECTION [J].
BERGAMINI, TM ;
PEYTON, JC ;
CHEADLE, WG .
JOURNAL OF SURGICAL RESEARCH, 1992, 52 (02) :101-105
[4]  
BERGAMINI TM, 1995, ARCH SURG-CHICAGO, V130, P1345
[5]   PREVENTION OF GRAFT INFECTION BY USE OF PROSTHESES BONDED WITH A RIFAMPIN/COLLAGEN RELEASE SYSTEM [J].
CHERVU, A ;
MOORE, WS ;
GELABERT, HA ;
COLBURN, MD ;
CHVAPIL, M .
JOURNAL OF VASCULAR SURGERY, 1991, 14 (04) :521-525
[6]   THE CRITICAL RELATIONSHIP OF ANTIBIOTIC DOSE AND BACTERIAL-CONTAMINATION IN EXPERIMENTAL-INFECTION [J].
CITAK, MS ;
CUE, JI ;
PEYTON, JC ;
MALANGONI, MA .
JOURNAL OF SURGICAL RESEARCH, 1992, 52 (02) :127-130
[7]   The emergence of mupirocin resistance: a challenge to infection control and antibiotic prescribing practice [J].
Cookson, BD .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1998, 41 (01) :11-18
[8]   VANCOMYCIN PENETRATION INTO BIOFILM COVERING INFECTED PROSTHESES AND EFFECT ON BACTERIA [J].
DAROUICHE, RO ;
DHIR, A ;
MILLER, AJ ;
LANDON, GC ;
RAAD, II ;
MUSHER, DM .
JOURNAL OF INFECTIOUS DISEASES, 1994, 170 (03) :720-723
[9]   Interpretive criteria for testing susceptibility of staphylococci to mupirocin [J].
Finlay, JE ;
Miller, LA ;
Poupard, JA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (05) :1137-1139
[10]   PSEUDOMONIC ACID - ANTIBIOTIC PRODUCED BY PSEUDOMONAS-FLUORESCENS [J].
FULLER, AT ;
MELLOWS, G ;
WOOLFORD, M ;
BANKS, GT ;
BARROW, KD ;
CHAIN, EB .
NATURE, 1971, 234 (5329) :416-&