A microplate-based method for real-time monitoring of Saccharomyces cerevisiae viability

被引：1

作者：

Ye, Yan-Rui ^{[1
]}

Ye, Mei-Li ^{[1
]}

Li, Li-Li ^{[1
]}

Lin, Ying ^{[1
]}

机构：

[1] S China Univ Technol, Sch Biosci & Bioengn, Guangzhou, Guangdong, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 405卷 / 01期

关键词：

YEAST VIABILITY; FLOW-CYTOMETRY; ASSAYS;

D O I：

10.1016/j.ab.2010.05.009

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Measuring the viability of the cells after chemical or physical stresses is a routine method in the determination of phenotypes in yeast. Numerous analytical techniques have been applied to the measurement of yeast cell viability, but few of them are suitable for real-time monitoring. Here we propose a microplate-based method for real-time monitoring of Saccharomyces cerevisiae cell viability. This method is also suitable for high-throughput measurement. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：144 / 146

页数：3

共 7 条

[1]

Detection of plant-modulated alterations in antifungal gene expression in Pseudomonas fluorescens CHA0 on roots by flow cytometry [J].