Study on noncovalent complexes of cytotoxic protoberberine alkaloids with double-stranded DNA by using electrospray ionization mass spectrometry

被引:47
作者
Chen, WH
Chan, CL
Cai, ZW
Luo, GA
Jiang, ZH [1 ]
机构
[1] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
[2] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Peoples R China
[3] Hong Kong Baptist Univ, Dept Chem, Kowloon, Hong Kong, Peoples R China
[4] Tsing Hua Univ, Anal Ctr, Beijing 100084, Peoples R China
关键词
D O I
10.1016/j.bmcl.2004.07.037
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The noncovalent complexes of four cytotoxic protoberberine alkaloids that is, berberine, palmatine, jatrorrhizine, and coptisine with double-stranded oligodeoxymicleotides d(AAGAATTCTT)(2) were investigated by electrospray ionization mass spectrometry. These four active components from Chinese herbal medicines showed both 1:1 and 1:2 binding stoichiometries, independent on the alkaloid-to-DNA ratios. Binding affinities in the order of palmatine greater than or equal to jatrorrhizine > coptisine > berberine with d(AAGAATTCTT)(2) were obtained. Additionally, the preliminary results indicated that berberine had some sequence selectivities. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4955 / 4959
页数:5
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