Study on noncovalent complexes of cytotoxic protoberberine alkaloids with double-stranded DNA by using electrospray ionization mass spectrometry
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作者:
Chen, WH
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机构:Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
Chen, WH
Chan, CL
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机构:Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
Chan, CL
Cai, ZW
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机构:Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
Cai, ZW
Luo, GA
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机构:Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
Luo, GA
Jiang, ZH
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Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R ChinaHong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
Jiang, ZH
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机构:
[1] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
[2] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Peoples R China
[3] Hong Kong Baptist Univ, Dept Chem, Kowloon, Hong Kong, Peoples R China
[4] Tsing Hua Univ, Anal Ctr, Beijing 100084, Peoples R China
The noncovalent complexes of four cytotoxic protoberberine alkaloids that is, berberine, palmatine, jatrorrhizine, and coptisine with double-stranded oligodeoxymicleotides d(AAGAATTCTT)(2) were investigated by electrospray ionization mass spectrometry. These four active components from Chinese herbal medicines showed both 1:1 and 1:2 binding stoichiometries, independent on the alkaloid-to-DNA ratios. Binding affinities in the order of palmatine greater than or equal to jatrorrhizine > coptisine > berberine with d(AAGAATTCTT)(2) were obtained. Additionally, the preliminary results indicated that berberine had some sequence selectivities. (C) 2004 Elsevier Ltd. All rights reserved.