Triacylglycerol biosynthesis and gene expression in microspore-derived cell suspension cultures of oilseed rape

被引:22
作者
Weselake, RJ [1 ]
Byers, SD
Davoren, JM
Laroche, A
Hodges, DM
Pomeroy, MK
Furukawa-Stoffer, TL
机构
[1] Univ Lethbridge, Dept Chem & Biochem, Lethbridge, AB T1K 3M4, Canada
[2] Agr Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada
[3] Agr Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada
关键词
triacylglycerol; Brassica; sucrose; acyltransferase; differential display;
D O I
10.1093/jexbot/49.318.33
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of sucrose concentration on triacylglycerol biosynthesis and associated gene expression was examined in a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv. Jet Neuf), The triacylglycerol content of the cells increased about 5-fold on a fresh weight basis when the sucrose concentration in the growth medium was raised from 2% to 22% (w/v), The specific activity of microsomal diacylglycerol acyltransferase (EC 2.3.1.20) and its activity per unit fresh weight increased about 2.5-fold and 6-fold, respectively, when sucrose concentration was increased from 2% to 14%, mRNA encoding the major oleosin also appeared to increase in abundance over the 2-14% sucrose concentration range when RNA fractions were analysed by the reverse transcription-polymerase chain reaction, The sucrose-mediated increases in diacylglycerol acyltransferase activity and oleosin mRNA indicated that the cell suspension could be a useful research tool for the identification of cDNAs encoding triacylglycerol biosynthetic enzymes and associated proteins.
引用
收藏
页码:33 / 39
页数:7
相关论文
共 39 条
[1]  
ATTREE SM, 1992, PLANTA, V187, P395, DOI 10.1007/BF00195664
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   FATTY-ACID COMPOSITION OF LEAF LIPIDS DETERMINED AFTER COMBINED DIGESTION AND FATTY-ACID METHYL-ESTER FORMATION FROM FRESH TISSUE [J].
BROWSE, J ;
MCCOURT, PJ ;
SOMERVILLE, CR .
ANALYTICAL BIOCHEMISTRY, 1986, 152 (01) :141-145
[4]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[5]  
Christie W.W., 1992, INFORM, V3, P1031
[6]   THE EFFECTS OF DIFFERENT CULTURAL CONDITIONS ON THE ACCUMULATION OF DEPOT LIPIDS NOTABLY PETROSELINIC ACID DURING SOMATIC EMBRYOGENESIS IN DAUCUS-CAROTA L [J].
DUTTA, PC ;
APPELQVIST, LA .
PLANT SCIENCE, 1989, 64 (02) :167-177
[7]   SOLUBILIZATION, PARTIAL-PURIFICATION AND PROPERTIES OF ACYL-COA - GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE FROM AVOCADO (PERSEA-AMERICANA) FRUIT MESOCARP [J].
ECCLESTON, VS ;
HARWOOD, JL .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1995, 1257 (01) :1-10
[8]   ABSCISIC-ACID OR HIGH OSMOTICUM PROMOTE ACCUMULATION OF LONG-CHAIN FATTY-ACIDS IN DEVELOPING EMBRYOS OF BRASSICA-NAPUS [J].
FINKELSTEIN, R ;
SOMERVILLE, C .
PLANT SCIENCE, 1989, 61 (02) :213-217
[9]   USE OF TRANSGENIC PLANTS AND MUTANTS TO STUDY THE REGULATION AND FUNCTION OF LIPID-COMPOSITION [J].
GIBSON, S ;
FALCONE, DL ;
BROWSE, J ;
SOMERVILLE, C .
PLANT CELL AND ENVIRONMENT, 1994, 17 (05) :627-637
[10]   LIPID EXTRACTION OF TISSUES WITH A LOW-TOXICITY SOLVENT [J].
HARA, A ;
RADIN, NS .
ANALYTICAL BIOCHEMISTRY, 1978, 90 (01) :420-426