Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap

被引：94

作者：

Green, JJ ^{[1
]}

Ying, LM ^{[1
]}

Klenerman, D ^{[1
]}

Balasubramanian, S ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 13期

关键词：

D O I：

10.1021/ja029149w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The kinetics of opening of the DNA quadruplex formed by the human telomeric repeat have been investigated using real-time fluorescence resonance energy transfer (FRET) measurements with a peptide nucleic acid (PNA) trap. It has been found that this opening is zero-order with respect to PNA, indicating that the initial step is a rate-limiting internal rearrangement of the quadruplex. A study of the temperature dependence of the rate of quadruplex opening was performed and the activation energy of the process estimated to be 98 +/- 8 kJ mol(-1).

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页码：3763 / 3767

页数：5

相关论文

共 34 条

[1] Functional and dysfunctional roles of quadruplex DNA in cells [J].

Arthanari, H ;

Bolton, PH .

CHEMISTRY & BIOLOGY, 2001, 8 (03) :221-230

[2]

BALAGURUMOORTHY P, 1994, J BIOL CHEM, V269, P21858

[3] Kinetics of conformational fluctuations in DNA hairpin-loops [J].

Bonnet, G ;

Krichevsky, O ;

Libchaber, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (15) :8602-8606

[4] Hybridization of PNA to structured DNA targets: Quadruplex invasion and the overhang effect [J].

Datta, B ;

Armitage, BA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (39) :9612-9619

[5] Conformational transitions monitored for single molecules in solution [J].

Edman, L ;

Mets, U ;

Rigler, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6710-6715

[6] PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES [J].

EGHOLM, M ;

BUCHARDT, O ;

CHRISTENSEN, L ;

BEHRENS, C ;

FREIER, SM ;

DRIVER, DA ;

BERG, RH ;

KIM, SK ;

NORDEN, B ;

NIELSEN, PE .

NATURE, 1993, 365 (6446) :566-568

[7] Effect of DNA secondary structure on human telomerase activity [J].

Fletcher, TM ;

Sun, DK ;

Salazar, M ;

Hurley, LH .

BIOCHEMISTRY, 1998, 37 (16) :5536-5541

[8] CYTOSINE CYTOSINE+ BASE-PAIRING STABILIZES DNA QUADRUPLEXES AND CYTOSINE METHYLATION GREATLY ENHANCES THE EFFECT [J].

HARDIN, CC ;

CORREGAN, M ;

BROWN, BA ;

FREDERICK, LN .

BIOCHEMISTRY, 1993, 32 (22) :5870-5880

[9] CATION-DEPENDENT TRANSITION BETWEEN THE QUADRUPLEX AND WATSON-CRICK HAIRPIN FORMS OF D(CGCG3GCG) [J].

HARDIN, CC ;

WATSON, T ;

CORREGAN, M ;

BAILEY, C .

BIOCHEMISTRY, 1992, 31 (03) :833-841

[10] Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique [J].

Jensen, KK ;

Orum, H ;

Nielsen, PE ;

Norden, B .

BIOCHEMISTRY, 1997, 36 (16) :5072-5077

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