Serine protease EpiP from Staphylococcus epidermidis catalyzes the processing of the epidermin precursor peptide

被引：54

作者：

Geissler, S ^{[1
]}

Gotz, F ^{[1
]}

Kupke, T ^{[1
]}

机构：

[1] UNIV TUBINGEN,D-72076 TUBINGEN,GERMANY

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 01期

关键词：

D O I：

10.1128/jb.178.1.284-288.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The function of serine protease EpiP in epidermin biosynthesis was investigated. Epidermin is synthesized as a 52-amino-acid precursor peptide, EpiA, which is posttranslationally modified and processed to the mature 22-amino acid peptide antibiotic, epiP was expressed in Staphylococcus carnosus with xylose-regulated expression vector pCX15, The cleavage of the unmodified EpiA precursor peptide to leader peptide and proepidermin by EpiP-containing culture filtrates of S. carnosus (pCX15epiP) was followed by reversed-phase chromatography and subsequent electrospray mass spectrometry.

引用

收藏

页码：284 / 288

页数：5

相关论文

共 43 条

[1] GENETIC-ANALYSIS OF EPIDERMIN BIOSYNTHETIC GENES AND EPIDERMIN-NEGATIVE MUTANTS OF STAPHYLOCOCCUS-EPIDERMIDIS [J].

AUGUSTIN, J ;

ROSENSTEIN, R ;

WIELAND, B ;

SCHNEIDER, U ;

SCHNELL, N ;

ENGELKE, G ;

ENTIAN, KD ;

GOTZ, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 204 (03) :1149-1154

[2]

AUGUSTIN J, 1991, NISIN NOVEL LANTIBIO, P277

[3]

BANERJEE S, 1988, J BIOL CHEM, V263, P9508

[4] CLONING, SEQUENCING AND PRODUCTION OF THE LANTIBIOTIC MERSACIDIN [J].

BIERBAUM, G ;

BROTZ, H ;

KOLLER, KP ;

SAHL, HG .

FEMS MICROBIOLOGY LETTERS, 1995, 127 (1-2) :121-126

[5]

BUCHMAN GW, 1988, J BIOL CHEM, V263, P16260

[6] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].

CHEN, EY ;

SEEBURG, PH .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170

[7] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI BY HIGH-VOLTAGE ELECTROPORATION [J].

DOWER, WJ ;

MILLER, JF ;

RAGSDALE, CW .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6127-6145

[8] GENETIC-STRUCTURE OF THE ENTEROCOCCUS-FAECALIS PLASMID PAD1-ENCODED CYTOLYTIC TOXIN SYSTEM AND ITS RELATIONSHIP TO LANTIBIOTIC DETERMINANTS [J].

GILMORE, MS ;

SEGARRA, RA ;

BOOTH, MC ;

BOGIE, CP ;

HALL, LR ;

CLEWELL, DB .

JOURNAL OF BACTERIOLOGY, 1994, 176 (23) :7335-7344

[9] IMPROVEMENTS OF PROTOPLAST TRANSFORMATION IN STAPHYLOCOCCUS-CARNOSUS [J].

GOTZ, F ;

SCHUMACHER, B .

FEMS MICROBIOLOGY LETTERS, 1987, 40 (2-3) :285-288

[10] PRESENCE OF DEHYDROALANINE IN ANTIBIOTIC NISIN AND ITS RELATIONSHIP TO ACTIVITY [J].

GROSS, E ;

MORELL, JL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1967, 89 (11) :2791-&

← 1 2 3 4 5 →