Qualification and performance characteristics of a quantitative enzyme-linked immunosorbent assay for human IgG antibodies to anthrax lethal factor antigen

被引：7

作者：

Selinsky, Cheryl L.

Whitlow, Vanessa D.

Smith, Larry R.

Kaslow, David C.

Horton, Holly M.

机构：

[1] Vical Incorp, San Diego, CA 92121 USA

[2] Arizona State Univ, Biodesign Inst, Tempe, AZ USA

来源：

BIOLOGICALS | 2007年 / 35卷 / 02期

关键词：

bioterrorism; anthrax; lethal factor; ELISA;

D O I：

10.1016/j.biologicals.2006.07.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The contribution of Bacillus anthracis lethal factor (LF)-specific immune responses to protection against anthrax disease in humans remains incompletely defined due, in part, to a paucity of qualified reagents and a lack of standardized serological assays. Toward this end, we have identified and characterized suitable positive quality control and standard reference sera and developed, optimized, and qualified an enzyme-linked immunosorbent assay (ELISA) to measure LF-binding IgG. Herein we describe the performance characteristics of this ELISA and propose criteria for its use in the detection and quantification of anti-LF IgG in human serum. (c) 2006 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

引用

页码：123 / 129

页数：7

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