3-dimensional imaging of collagen using second harmonic generation

被引：361

作者：

Cox, G ^{[1
]}

Kable, E

Jones, A

Fraser, IK

Manconi, F

Gorrell, MD

机构：

[1] Univ Sydney, Electron Microscope Unit, Sydney, NSW 2006, Australia

[2] Univ Sydney, Dept Obstet & Gynaecol, Sydney, NSW 2006, Australia

[3] Univ Sydney, Dept Med, AW Morrow Gastroenterol & Liver Ctr, Sydney, NSW 2006, Australia

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2003年 / 141卷 / 01期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

second harmonic generation; collagen; 3D reconstruction; cirrhosis;

D O I：

10.1016/S1047-8477(02)00576-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Collagen is the most important structural protein of the animal body. Its unique triple-helix structure and extremely high level of crystallinity make it exceptionally efficient in generating the second harmonic of incident light, and we show here how this leads to a novel mode of microscopy of immediate practical significance in medicine and biology. In particular, it provides sensitive and high-resolution information on collagen distribution, discriminates between type I and type III collagen, and allows both a greater understanding of and a sensitive test for cirrhosis of the liver. Future research applications could include wound healing and hereditary collagen diseases such as osteogenesis imperfecta. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：53 / 62

页数：10

共 27 条

[1] Extracellular matrix degradation and the role of hepatic stellate cells [J].

Benyon, RC ;

Arthur, MJP .

SEMINARS IN LIVER DISEASE, 2001, 21 (03) :373-384

[2]

Born M., 1993, PRINCIPLES OPTICS

[3] PROBING MEMBRANE-POTENTIAL WITH NONLINEAR OPTICS [J].

BOUEVITCH, O ;

LEWIS, A ;

PINEVSKY, I ;

WUSKELL, JP ;

LOEW, LM .

BIOPHYSICAL JOURNAL, 1993, 65 (02) :672-679

[4]

BRADBURY S, 1973, PEACOCKS ELEMENTARY

[5] Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues [J].

Campagnola, PJ ;

Millard, AC ;

Terasaki, M ;

Hoppe, PE ;

Malone, CJ ;

Mohler, WA .

BIOPHYSICAL JOURNAL, 2002, 82 (01) :493-508

[6] Second-harmonic imaging microscopy of living cells [J].

Campagnola, PJ ;

Clark, HA ;

Mohler, WA ;

Lewis, A ;

Loew, LM .

JOURNAL OF BIOMEDICAL OPTICS, 2001, 6 (03) :277-286

[7] Laser-scanning coherent anti-stokes Raman scattering microscopy and applications to cell biology [J].

Cheng, JX ;

Jia, YK ;

Zheng, GF ;

Xie, XS .

BIOPHYSICAL JOURNAL, 2002, 83 (01) :502-509

[8] Second harmonic imaging of collagen in mammalian tissue [J].

Cox, G ;

Manconi, F ;

Kable, E .

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES II, 2002, 4620 :148-156

[9]

DZIEDZICGOCLAWSKA A, 1982, BASIC APPL HISTOCHEM, V26, P227

[10] GENERATION OF OPTICAL HARMONICS [J].

FRANKEN, PA ;

WEINREICH, G ;

PETERS, CW ;

HILL, AE .

PHYSICAL REVIEW LETTERS, 1961, 7 (04) :118-&

← 1 2 3 →