Elevated peripheral benzodiazepine receptor expression in simian immunodeficiency virus encephalitis

Measurement of central nervous system (CNS) expression of the peripheral benzodiazepine receptor (PBR), a microglia and macrophage activation marker, by positron emission tomography (PET) would aid clinical management of human immunodeficiency virus (HIV)-infected patients. To evaluate the utility of examining PBR expression in the CNS as a cellular activation marker in HIV CNS disease, PBR levels were measured in frontal cortex of simian immunodeficiency virus (SIV)-infected macaques with encephalitis and uninfected animals via PK11195 ligand autoradiography. [H-3]-(R) PK11195 binding to both grey matter ( P =.017) and white matter ( P =.038) was significantly higher in animals with SIV encephalitis (n=10) versus control animals (n=3). When PK11195 binding was compared with other microglial/ macrophage activation markers (obtained via quantitative image analysis), a strong, significant association was found for both HAM56 ( P =.004) and KP-1 (anti-CD68; P =.006) immunostaining in white matter. In contrast, grey matter PK11195 binding did not correlate with HAM56 (P =.46), KP-1 (P =.06), or glial fibrillary acidic protein (GFAP) immunostaining for astrocytic activation ( P =.09). The regional nature of these increases in activation within the brain illustrates the crucial need to focus functional neuroimaging analyses of HIV-infected individuals on subcortical white matter to assess activation of microglia and macrophages.