Rapid and simple determination of the Escherichia coli phylogenetic group

被引:2303
作者
Clermont, O [1 ]
Bonacorsi, S [1 ]
Bingen, E [1 ]
机构
[1] Univ Paris 07, Microbiol Serv, Hop Robert Debre, Lab Etud Genet Bacterienne Infect Enfant,EA3105, F-75395 Paris 19, France
关键词
D O I
10.1128/AEM.66.10.4555-4558.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on tripler PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
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收藏
页码:4555 / 4558
页数:4
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