Rapid and simple determination of the Escherichia coli phylogenetic group

被引：2303

作者：

Clermont, O ^{[1
]}

Bonacorsi, S ^{[1
]}

Bingen, E ^{[1
]}

机构：

[1] Univ Paris 07, Microbiol Serv, Hop Robert Debre, Lab Etud Genet Bacterienne Infect Enfant,EA3105, F-75395 Paris 19, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 10期

关键词：

D O I：

10.1128/AEM.66.10.4555-4558.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on tripler PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.

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页码：4555 / 4558

页数：4

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