Prioritization of Competing Damage and Developmental Signals by Migrating Macrophages in the Drosophila Embryo

被引:163
作者
Moreira, Severina [1 ,2 ]
Stramer, Brian [3 ]
Evans, Iwan [4 ]
Wood, Will [4 ]
Martin, Paul [1 ,2 ]
机构
[1] Univ Bristol, Dept Biochem, Bristol BS8 1TD, Avon, England
[2] Univ Bristol, Dept Physiol & Pharmacol, Bristol BS8 1TD, Avon, England
[3] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England
[4] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
基金
英国医学研究理事会; 英国惠康基金;
关键词
CELL-MIGRATION; HYDROGEN-PEROXIDE; BLOOD-CELLS; INFLAMMATION; CHEMOTAXIS; MOUSE; MELANOGASTER; NAVIGATION; ZEBRAFISH; HEMOCYTES;
D O I
10.1016/j.cub.2010.01.047
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The function of immune cells is critically dependent on their capacity to respond to a complex series of navigational cues that enable them to home to various organ sites in the body or to respond to inflammatory cues such as those released at sites of tissue damage. From early embryonic stages, immune cells are faced with a barrage of signals that will not all be directing the cell to do the same thing. Here we use the Drosophila embryo to investigate how hemocytes (Drosophila macrophages), are able to prioritize key guidance signals and ignore others so that they are not pulled every which way. We identify the immediate wound attractant signal as H2O2 and investigate how Drosophila macrophages respond to competing guidance cues-those emanating from a wound-versus standard developmental guidance cues, as well as those signals drawing cells toward neighboring dying cells. We reveal a hierarchy of responsiveness to attractant cues that varies over time and we identify why there is a wound refractile period early in embryonic development when macrophages cannot be distracted from their developmental migratory pathway to a site of tissue damage.
引用
收藏
页码:464 / 470
页数:7
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