Protein-protein interaction site mapping using NMR-detected mutational scanning

被引：6

作者：

Baminger, Bettina

Ludwiczek, Martin L.

Kontaxis, Georg

Knapp, Stefan

Konrat, Robert ^{[1
]}

机构：

[1] Univ Vienna, Max F Perutz Labs, Dept Biomol Struct Chem, A-1030 Vienna, Austria

[2] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC, Canada

[3] Univ Oxford, Botnar Res Ctr, SGC, Oxford OX3 7LD, England

来源：

JOURNAL OF BIOMOLECULAR NMR | 2007年 / 38卷 / 02期

关键词：

mutagenesis; NMR spectroscopy; Protein Interaction; proteomics;

D O I：

10.1007/s10858-007-9154-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.

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收藏

页码：133 / 137

页数：5

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