Regulatory mechanism of the initiation step of DNA replication by CDK in budding yeast

被引：9

作者：

Araki, Hiroyuki ^{[1
,2
]}

机构：

[1] Res Org Informat & Syst, Natl Inst Genet, Div Microbial Genet, Shizuoka, Japan

[2] SOKENDAI, Dept Genet, Shizuoka, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2010年 / 1804卷 / 03期

关键词：

CDK; DNA-replication; Eukaryotes; Yeast; Multiple phosphorylation; S-PHASE; DEPENDENT PHOSPHORYLATION; SACCHAROMYCES-CEREVISIAE; EUKARYOTIC CELLS; SLD2; PROTEIN; DPB11; INTERACTS; CDC7;

D O I：

10.1016/j.bbapap.2009.10.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclin-dependent kinases (CDKs) regulate the progression of the cell cycle in eukaryotes. At the onset of chromosomal DNA replication, CDKs phosphorylate two replication proteins, Sld2 and Sld3, in budding yeast. Phosphorylated Sld2 and Sld3 enhance the formation of complexes with the BRCT (BRCA1 C-terminal)containing replication protein Dpb11. The formation of these complexes is essential and sufficient for the CDK-dependent activation of the initiation of chromosomal DNA replication. Multiple phosphorylation of Sld2 by CDKs fine-tunes the process of complex formation. Here, we discussed the regulation of the initiation step of chromosomal DNA replication via CDK-dependent phosphorylation. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：520 / 523

页数：4

共 15 条

[1] DPB11, WHICH INTERACTS WITH DNA-POLYMERASE II(EPSILON) IN SACCHAROMYCES-CEREVISIAE, HAS A DUAL ROLE IN S-PHASE PROGRESSION AND AT A CELL-CYCLE CHECKPOINT [J].