Surface plasmon resonance biosensor for enrofloxacin based on deoxyribonucleic acid

被引：29

作者：

Cao, Limin

Lin, Hong

Mirsky, Vladimir M. ^{[1
]}

机构：

[1] Univ Regensburg, Inst Analyt Chem Cheno & Biosensors, D-266003 Regensburg, Germany

[2] Ocean Univ China, Aquat Prod Safety Lab, Qingdao 266003, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 589卷 / 01期

基金：

中国国家自然科学基金;

关键词：

surface plasmon resonance; nucleic acid; biosensor; enrofloxacin; fluoroquinolones; layer-by-layer deposition;

D O I：

10.1016/j.aca.2007.02.034

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A DNA-based surface plasmon resonance biosensor for enrofloxacin was developed. Heating denatured DNA immobilized on the gold-coated glass surface was exploited. The immobilization was performed by a layer-by-layer co-deposition with a cationic polymer. The sensor performance was tested with real biological probes. Direct and simple determination of enrofloxacin in milk samples was demonstrated. The sensor response obeys Langmuir binding isotherm being almost linear until about 20 mu g mL(-1). The detection limit in milk samples was estimated to be 3 mu g mL(-1). (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1 / 5

页数：5

共 29 条

[1] Interaction between DNA gyrase and quinolones:: Effects of alanine mutations at GyrA subunit residues Ser83 and Asp87 [J].