Apoptosis induced by penta-acetyl geniposide in C6 glioma cells is associated with JNK activation and Fas ligand induction

In our previous study, penta-acetyl geniposide ((AC)(5)GP) is suggested to induce tumor cell apoptosis through the specific activation of PKCdelta. However, the downstream signal pathway of PKCdelta has not yet been investigated. It was shown that JNK may play an important role in the regulation of apoptosis and could be a possible downstream signal of PKCdelta isoforms. In the present study, we investigate whether JNK is involved in (AC)(5)GP induced apoptosis. The result reveals that (AC)(5)GP induces JNK activation and c-Jun phosphorylation thus stimulating the expression of Fas-L and Fas. Using SP600125 to block JNK activation shows that (AC)(5)GP-mediated apoptosis and related proteins expression are attenuated. Furthermore, we find that the (AC)(5)GP induces apoptosis through the activation of JNK/Jun/Fas L/Fas/ caspase 8/caspase 3, a mitochondria-independent pathway. The JNK pathway is suggested to be the downstream signal of PKCdelta, since rottlerin impedes (AC)(5)GP-induced JNK activation. Therefore, (AC)(5)GP mediates cell death via activation of PKCdelta/JNK/FasL cascade signaling. (C) 2004 Elsevier Inc. All rights reserved.