Detection rates of TT virus DNA in serum of umbilical cord blood, breast milk and saliva

被引:37
作者
Goto, K [1 ]
Sugiyama, K
Ando, T
Mizutani, F
Terabe, K
Tanaka, K
Nishiyama, M
Wada, Y
机构
[1] Nagoya City Univ, Sch Med, Dept Pediat, Mizuho Ku, Nagoya, Aichi 4678601, Japan
[2] Yonaha Gen Hosp, Dept Gynecol, Kuwana 5110838, Japan
关键词
polymerase chain reaction; pregnant women; horizontal route of transmission;
D O I
10.1620/tjem.191.203
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To date, the routes of mother-to-infant transmission of TT virus (TTV) have not been fully elucidated. The present study examines the detection rates of TTV DNA. in the serum of pregnant Japanese women and in cord blood at the time of delivery, as well as in the saliva and breast milk of mothers one-month postpartum. Primers derived from the well-known translated region N22 (N22 system), as well as the untranslated region (UTR system) were used. The prevalence of TTV DNA in the serum of pregnant women was found to be 11.9% (19/160) using the N22 system and 72.4% (55/76) using the UTR system. No TTV DNA was detected in the cord blood samples (0/160) when the N22 system was used for detection but TTV DNA was detected in 11.8% (7/76) of samples studied with the UTR system. Using the N22 system, TTV DNA was not detected in breast milk, but was detected in saliva. However using the UTR system, TTV DNA was detected in both specimens. These results imply that some babies are vertically infected with TTV via cord blood at the time of delivery or via breast milk or saliva. However, further research is necessary to confirm this hypothesis.
引用
收藏
页码:203 / 207
页数:5
相关论文
共 23 条
[1]   Early acquisition of TT virus (TTV) in an area endemic for TTV infection [J].
Davidson, F ;
MacDonald, D ;
Mokili, JLK ;
Prescott, LE ;
Graham, S ;
Simmonds, P .
JOURNAL OF INFECTIOUS DISEASES, 1999, 179 (05) :1070-1076
[2]   TT virus: a study of molecular epidemiology and transmission of genotypes 1, 2 and 3 [J].
Gallian, P ;
Biagini, P ;
Zhong, S ;
Touinssi, M ;
Yeo, W ;
Cantaloube, JF ;
Attoui, H ;
de Micco, P ;
Johnson, PJ ;
de Lamballerie, X .
JOURNAL OF CLINICAL VIROLOGY, 2000, 17 (01) :43-49
[3]  
Inami T, 2000, J CLIN MICROBIOL, V38, P2407
[4]   High frequency of postnatal transmission of TT virus in infancy [J].
Kazi, A ;
Miyata, H ;
Kurokawa, K ;
Khan, MA ;
Kamahora, T ;
Katamine, S ;
Hino, S .
ARCHIVES OF VIROLOGY, 2000, 145 (03) :535-540
[5]   A comparison of amino acid sequences of hepatitis B virus S gene in 46 children presenting various clinical features for immunoprophylaxis [J].
Miyake, Y ;
Oda, T ;
Li, R ;
Sugiyama, K .
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 180 (03) :233-247
[6]   TT virus: Evidence for transplacental transmission [J].
Morrica, A ;
Maggi, F ;
Vatteroni, ML ;
Fornai, C ;
Pistello, M ;
Ciccorossi, P ;
Grassi, E ;
Gennazzani, A ;
Bendinelli, M .
JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (02) :803-804
[7]   Molecular and biophysical characterization of TT virus: Evidence for a new virus family infecting humans [J].
Mushahwar, IK ;
Erker, JC ;
Muerhoff, AS ;
Leary, TP ;
Simons, JN ;
Birkenmeyer, LG ;
Chalmers, ML ;
Pilot-Matias, TJ ;
Dexai, SM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :3177-3182
[8]   A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology [J].
Nishizawa, T ;
Okamoto, H ;
Konishi, K ;
Yoshizawa, H ;
Miyakawa, Y ;
Mayumi, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 241 (01) :92-97
[9]   Marked genomic heterogeneity and frequent mixed infection of TT virus demonstrated by PCR with primers from coding and noncoding regions [J].
Okamoto, H ;
Takahashi, M ;
Nishizawa, T ;
Ukita, M ;
Fukuda, M ;
Tsuda, F ;
Miyakawa, Y ;
Mayumi, M .
VIROLOGY, 1999, 259 (02) :428-436
[10]   Molecular cloning and characterization of a novel DNA virus (TTV) associated with posttransfusion hepatitis of unknown etiology [J].
Okamoto, H ;
Nishizawa, T ;
Kato, N ;
Ukita, M ;
Ikeda, H ;
Iizuka, H ;
Miyakawa, Y ;
Mayumi, M .
HEPATOLOGY RESEARCH, 1998, 10 (01) :1-16