Epinephrine modulates cellular distribution of muscle phosphofructokinase

被引：39

作者：

Alves, GG

Sola-Penna, M ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Fac Farm, Dept Farmacos, Lab Enzimol & Controle Metab, Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Dept Bioquim Med, Rio De Janeiro, Brazil

来源：

MOLECULAR GENETICS AND METABOLISM | 2003年 / 78卷 / 04期

关键词：

phosphofructokinase; epinephrine; cellular distribution; muscle; actin;

D O I：

10.1016/S1096-7192(03)00037-4

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In this paper, we report evidences that cellular distribution of phosphofructokinase can be affected by epinephrine stimulation in rabbit skeletal muscle homogenates. Through co-sedimentation techniques, we observed that in epinephrine-stimulated tissues, approximately 50% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 29% in control. This phenomenon is companied by a 400% increase in specific phosphofructokinase activity in stimulated homogenates. This effect is reproduced by the beta-adrenergic agonist isoprenaline. Here we propose that the modulation of cellular distribution of phosphofructokinase may be one of the mechanisms of control of glycolytic flux in mammalian muscle, by beta-adrenergic stimulation. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：302 / 306

页数：5

共 37 条

[1] Myofibril-bound muscle phosphofructokinase is less sensitive to inhibition by ATP than the free enzyme, but retains its sensitivity to stimulation by bisphosphorylated hexoses [J].