Expression of a cascading genetic network within liposomes

被引：163

作者：

Ishikawa, K

Sato, K

Shima, Y

Urabe, I

Yomo, T

机构：

[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Informat Sci & Technol, Dept Bioinformat Engn, Suita, Osaka 5650871, Japan

[3] Univ Tokyo, Dept Pure & Appl Sci, Meguro Ku, Tokyo 1538902, Japan

[4] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[5] ERATO, ZST, Tokyo, Japan

来源：

FEBS LETTERS | 2004年 / 576卷 / 03期

关键词：

liposome; genetic network; cell-free protein synthesis; T7 RNA polymerase; GFP; flow cytometry;

D O I：

10.1016/j.febslet.2004.09.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liposomes have long been used as possible compartments for artificial cells, and it has been shown that liposomes can sustain various types of biochemical reactions. To elevate the degree of molecular complexity of the system in liposomes, we have constructed a two-stage genetic network encapsulated in liposomes. This two-stage genetic network was constructed with the plasmid pTH, in which the protein product of the first stage (T7 RNA polymerase) is required to drive the protein synthesis of the second stage (GFP). We show that the two-stage genetic network constructed in a cell-free expression system is functional within liposomes. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：387 / 390

页数：4

共 22 条

[1] IDENTIFICATION AND CHARACTERIZATION OF RECD, A GENE AFFECTING PLASMID MAINTENANCE AND RECOMBINATION IN ESCHERICHIA-COLI [J].