Production of interleukin-12 and expression of its receptors by murine microglia

被引：48

作者：

Suzumura, A

Sawada, M

Takayanagi, T

机构：

[1] Nara Med Univ, Dept Neurol, Kashihara, Nara 634, Japan

[2] Fujita Hlth Univ, Sch Med, Inst Comprehens Med Sci, Div Pathol & Biochem, Toyoake, Aichi 47011, Japan

来源：

BRAIN RESEARCH | 1998年 / 787卷 / 01期

关键词：

microglia; astrocyte; glia; cytokine; interleukin-12; helper T cell;

D O I：

10.1016/S0006-8993(97)01166-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Production of interleukin-12 (IL-12) by cultured murine microglia and astrocytes was examined, by means of ELISA to detect heterodimeric p70 and RT-PCR to analyze the expression of mRNA encoding p35 and p40. Microglia, but not astrocytes, produced IL-12 p70 in response to lipopolysaccharide and interferon-gamma. The microglial cell line, Ra2, produced only p40, but not p35, upon above stimulation. Thus, it is possible that some population of microglia induce helper 1 type T cell response via producing IL-12 in the CNS. Microglia were induced to express mRNA encoding IL-12 receptors which were exclusively expressed in activated T and NK cells. (C) 1998 Elsevier Science B.V.

引用

页码：139 / 142

页数：4

共 23 条

[1] Soluble tumor necrosis factor receptor inhibits interleukin 12 production by stimulated human adult microglial cells in vitro [J].