Paramagnetic NMR analysis of the seven-iron ferredoxin from the hyperthermoacidophilic archaeon Desulfurolobus ambivalens reveals structural similarity to other dicluster ferredoxins

被引:23
作者
Bentrop, D
Bertini, I
Luchinat, C
Mendes, J
Piccioli, M
Teixeira, M
机构
[1] UNIV FLORENCE,DEPT CHEM,I-50121 FLORENCE,ITALY
[2] UNIV BOLOGNA,INST AGR CHEM,I-40126 BOLOGNA,ITALY
[3] UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL,P-2780 OEIRAS,PORTUGAL
[4] UNIV NOVA LISBOA,FAC CIENCIAS & TECNOL,P-2780 OEIRAS,PORTUGAL
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 236卷 / 01期
关键词
NMR; ferredoxin; paramagnetic; Desulfurolobus ambivalens; hyperthermoacidophilic;
D O I
10.1111/j.1432-1033.1996.00092.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The seven-iron ferredoxin from the hyperthermophilic archaeon Desulfurolobus ambivalens has been investigated by one-dimensional and two-dimensional H-1-NMR in its oxidized and dithionite-reduced states. Al iron atoms of both the three-iron and the four-iron cluster are bound to cysteine residues whose hyperfine shifted resonances were characterized. The pattern of these resonances is similar to those from three-iron, four-iron and eight-iron ferredoxins previously described in the literature, but the four-iron cluster has a shift pattern different from that in other seven-iron proteins. A second set of hyperfine-shifted resonances clearly indicates sample heterogeneity, which possibly involves the four-iran cluster. The observation of interresidue NOEs between two different cysteine residues proves the existence of close spatial proximity of the two dusters in D. ambivalens ferredoxin and therefore indicates structural homology to other dicluster ferredoxins. Moreover, this feature is crucial for the sequence-specific assignment of the hyperfine-shifted resonances. The C alpha-C beta-S-Fe dihedral angles of the cysteine residues coordinating the four-iron cluster could be estimated, and the electronic structure of the three-iron cluster is discussed.
引用
收藏
页码:92 / 99
页数:8
相关论文
共 38 条
  • [1] H-1 NOE STUDIES ON DICOPPER(II) DICOBALT(II) SUPEROXIDE-DISMUTASE
    BANCI, L
    BERTINI, I
    LUCHINAT, C
    PICCIOLI, M
    SCOZZAFAVA, A
    TURANO, P
    [J]. INORGANIC CHEMISTRY, 1989, 28 (26) : 4650 - 4656
  • [2] MAGNETIC-RESONANCE OF FE-S CLUSTERS - ISOLATION AND CHARACTERIZATION OF A 7FE FERREDOXIN FROM RHODOPSEUDOMONAS-PALUSTRIS
    BATTISTUZZI, G
    BORSARI, M
    FERRETTI, S
    LUCHINAT, C
    SOLA, M
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 320 (01) : 149 - 154
  • [3] H-1-NMR STUDIES OF THE OXIDIZED AND PARTIALLY REDUCED 2(4FE-4S) FERREDOXIN FROM CLOSTRIDIUM-PASTEURIANUM
    BERTINI, I
    BRIGANTI, F
    LUCHINAT, C
    SCOZZAFAVA, A
    [J]. INORGANIC CHEMISTRY, 1990, 29 (10) : 1874 - 1880
  • [4] ARE TRUE SCALAR PROTON PROTON CONNECTIVITIES EVER MEASURED IN COSY SPECTRA OF PARAMAGNETIC MACROMOLECULES
    BERTINI, I
    LUCHINAT, C
    TARCHI, D
    [J]. CHEMICAL PHYSICS LETTERS, 1993, 203 (5-6) : 445 - 449
  • [5] SOLUTION STRUCTURE OF THE OXIDIZED 2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM-PASTEURIANUM
    BERTINI, I
    DONAIRE, A
    FEINBERG, BA
    LUCHINAT, C
    PICCIOLI, M
    YUAN, HP
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 232 (01): : 192 - 205
  • [6] THE FE4S4 CENTERS IN FERREDOXINS STUDIED THROUGH PROTON AND CARBON HYPERFINE COUPLING - SEQUENCE-SPECIFIC ASSIGNMENTS OF CYSTEINES IN FERREDOXINS FROM CLOSTRIDIUM-ACIDI-URICI AND CLOSTRIDIUM-PASTEURIANUM
    BERTINI, I
    CAPOZZI, F
    LUCHINAT, C
    PICCIOLI, M
    VILA, AJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (02) : 651 - 660
  • [7] BERTINI I, 1995, STRUCT BOND, V83, P1
  • [8] DOUBLE EXCHANGE VERSUS J INEQUALITY IN FE3S40 CLUSTERS
    BERTINI, I
    BRIGANTI, F
    LUCHINAT, C
    [J]. INORGANICA CHIMICA ACTA, 1990, 175 (01) : 9 - 10
  • [9] BUSSE SC, 1991, J BIOL CHEM, V266, P23714
  • [10] PROTON NMR INVESTIGATION OF THE OXIDIZED 3-IRON CLUSTERS IN THE FERREDOXINS FROM THE HYPERTHERMOPHILIC ARCHAE PYROCOCCUS-FURIOSUS AND THERMOCOCCUS-LITORALIS
    BUSSE, SC
    LAMAR, GN
    YU, LPP
    HOWARD, JB
    SMITH, ET
    ZHOU, ZH
    ADAMS, MWW
    [J]. BIOCHEMISTRY, 1992, 31 (47) : 11952 - 11962