Comparative study of poliovirus recovery techniques from mussel tissues

被引：4

作者：

Mignotte, B

Terver, D

Schwartzbrod, L

机构：

[1] Fac Pharm, Virol Lab, F-54001 Nancy, France

[2] Fac Sci, Aquarium Trop, F-54000 Nancy, France

来源：

MARINE POLLUTION BULLETIN | 1997年 / 34卷 / 11期

关键词：

poliovirus; mussels; artificial contamination; extraction; concentration; cell culture;

D O I：

10.1016/S0025-326X(97)00060-X

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Four poliovirus extraction and three poliovirus concentration techniques designed for the study of mussel tissues were compared. The recovered infectious viral particles were confirmed by inoculation to cell cultures. The protocol that provided optimum results involved extraction with a solution of NaCl 0.3 M/beef extract 7%/freon at pH 7.5, followed by a concentration with polyethylene glycol 6000. The resulting concentration rate ranged from 80 to 136%, The amount of infectious viral particles thus recovered was 1x10(5) MPNCU g(-1) of tissue (approximately) for an initial concentration of seawater of 1.5x10(3) MPNCU ml(-1), (C) 1997 Elsevier Science Ltd.

引用

收藏

页码：875 / 879

页数：5

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