Neurosteroids:: Expression of functional 3β-hydroxysteroid dehydrogenase by rat sensory neurons and Schwann cells

被引:62
作者
Guennoun, R
Schumacher, M
Robert, F
Delespierre, B
Gouézou, M
Eychenne, B
Akwa, Y
Rebel, P
Baulieu, EE
机构
[1] INSERM, U33, Lab Hormones, F-94276 Bicetre, France
[2] Coll France, Paris, France
关键词
development; dorsal root ganglia; pregnenolone; progesterone; regeneration;
D O I
10.1111/j.1460-9568.1997.tb01642.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Steroids which are synthesized within the nervous system, such as progesterone, have been termed 'neurosteroids'. Levels of progesterone are much larger in peripheral nerves of rats and mice than in plasma, and persist after removal of the steroidogenic endocrine glands. Schwann cells are a source of progesterone: when isolated from embryonic dorsal root ganglia, they can synthesize progesterone from pregnenolone, the obligate precursor of all steroids, Locally produced progesterone has been shown to play an important role in myelination of peripheral nerve. We show here that sensory neurons from embryonic dorsal root ganglia also express 3 beta-hydroxysteroid dehydrogenase and can convert [H-3]pregnenolone to [H-3]progesterone. Moreover, when cultured under different conditions and incubated for 24 h in the presence of 100 nM [H-3]pregnenolone, they produce 5-10 times more [H-3]progesterone than Schwann cells. The conversion of pregnenolone to progesterone by neurons is further increased by a diffusible factor produced by Schwann cells. Sensory neurons can also metabolize progesterone to 5 alpha-dihydroprogesterone, but unlike Schwann cells, they do not produce 3 alpha,5 alpha-tetrahydroprogesterone, a potent positive allosteric modulator of gamma-aminobutyric acid type A receptors. We also show that cells isolated from the adult nervous system still have the capacity to convert [H-3]pregnenolone to progesterone and its 5 alpha-reduced metabolites: neurons and Schwann cells purified from dorsal root ganglia of 6 week old male rats show a similar pattern of pregnenolone metabolism to cells isolated from 18 day old embryos. These findings further support the important role of progesterone in the development and regeneration of the peripheral nervous system.
引用
收藏
页码:2236 / 2247
页数:12
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