Degradation of pentaerythritol tetranitrate by Enterobacter cloacae PB2

被引:103
作者
Binks, PR
French, CE
Nicklin, S
Bruce, NC
机构
[1] UNIV CAMBRIDGE,INST BIOTECHNOL,CAMBRIDGE CB2 1QT,ENGLAND
[2] DEF RES AGCY,SEVENOAKS TN14 7BP,KENT,ENGLAND
关键词
D O I
10.1128/AEM.62.4.1214-1219.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A mixed microbial culture capable of metabolizing the explosive pentaerythritol tetranitrate (PETN) was obtained from soil enrichments under aerobic and nitrogen-limiting conditions. A strain of Enterobacter cloacae, designated PB2, was isolated from this culture and was found to use PETN as a sole source of nitrogen for growth, Growth yields suggested that 2 to 3 mol of nitrogen was utilized per mol of PETN, The metabolites pentaerythritol dinitrate, 3-hydroxy-2,2-bis-[(nitrooxy)methyl] propanal, and 2,2-bis-[(nitrooxy)methyl] -propanedial were identified by mass spectrometry and H-1-nuclear magnetic resonance. An NADPH-dependent PETN reductase was isolated from cell extracts and shown to liberate nitrite from PETN, producing pentaerythritol tri- and dinitrates which were identified by mass spectrometry. PETN reductase was purified to apparent homogeneity by ion-exchange and affinity chromatography. The purified enzyme was found to be a monomeric flavoprotein with a M(r) of approximately 40,000, binding flavin mononucleotide noncovalently.
引用
收藏
页码:1214 / 1219
页数:6
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