Comparative study of the cytotoxic and genotoxic effects of titanium oxide and aluminium oxide nanoparticles in Chinese hamster ovary (CHO-K1) cells

被引：150

作者：

Di Virgilio, A. L. ^{[1
]}

Reigosa, M. ^{[2
]}

Arnal, P. M. ^{[1
,3
]}

Fernandez Lorenzo de Mele, M. ^{[1
]}

机构：

[1] Inst Invest Fis Quim Teor & Aplicadas INIFTA, RA-1900 La Plata, Buenos Aires, Argentina

[2] Inst Multidisciplinario Biol Celular IMBICE, RA-1900 La Plata, Buenos Aires, Argentina

[3] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2010年 / 177卷 / 1-3期

关键词：

Titanium oxide; Aluminium oxide; Nanoparticles; Cytotoxicity; Genotoxicity; Cell culture; ULTRAFINE TIO2 PARTICLES; IN-VITRO CYTOTOXICITY; DIOXIDE NANOPARTICLES; OXIDATIVE STRESS; GOLD NANOPARTICLES; DIFFERENT PHASES; CARBON-BLACK; TOXICITY; DNA; ASSAYS;

D O I：

10.1016/j.jhazmat.2009.12.089

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The aim of this study was to analyze the cytotoxicity and genotoxicity of titanium oxide (TiO2) and aluminium oxide (Al2O3) nanoparticles (NPs) on Chinese hamster ovary (CHO-K1) cells using neutral red (NR), mitochondrial activity (by MTT assay), sister chromatid exchange (SCE), micronucleus (MN) formation, and cell cycle kinetics techniques. Results showed a dose-related cytotoxic effect evidenced after 24 h by changes in lysosomal and mitochondrial dehydrogenase activity. Interestingly, transmission electronic microscopy (TEM) showed the formation of perinuclear vesicles in CHO-K1 cells after treatment with both NPs during 24 h but no NP was detected in the nuclei. Genotoxic effects were shown by MN frequencies which significantly increased at 0.5 and 1 mu g/mL TiO2 and 0.5-10 mu g/mL Al2O3. SCE frequencies were higher for cells treated with 1-5 mu g/mL TiO2. The absence of metaphases evidenced cytotoxicity for higher concentrations of TiO2. No SCE induction was achieved after treatment with 1-25 mu g/mL Al2O3. In conclusion, findings showed cytotoxic and genotoxic effects of TiO2 and Al2O3 NPs on CHO-K1 cells. Possible causes of controversial reports are discussed further on. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：711 / 718

页数：8

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