Increased translation efficiency and antizyme-dependent stabilization of ornithine decarboxylase in amino acid-supplemented human colon adenocarcinoma cells, Caco-2

被引:13
作者
Chabanon, H
Persson, L
Wallace, HM
Ferrara, M
Brachet, P [1 ]
机构
[1] INRA, Unite Nutr Cellulaire & Mol, F-63122 Theix, France
[2] Univ Lund, Dept Physiol Sci, S-22362 Lund, Sweden
[3] Univ Aberdeen, Dept Med Therapeut & Biomed Sci, Inst Med Sci, Aberdeen AB25 2ZD, Scotland
关键词
glycine; polyamine biosynthesis; nutrient; ODC; post-translational regulation;
D O I
10.1042/0264-6021:3480401
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mechanisms of the response of ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis, to amino acid supplementation were studied in the human colon adenocarcinoma cell line, Caco-2. Supplementation of serum-deprived, subconfluent Caco-2 cells with any one of a series of amino acids (10 mM) resulted in increased ODC activity, reaching a maximum of approx. 12.5-fold after approx. 4 h, over control cells either not supplemented or supplemented with isoosmolar D-mannitol. Glycine, L-asparagine and L-serine, as well as their D-enantiomers, were the strongest effecters and acted in a concentration-dependent manner; millimolar concentrations of most of these amino acids being sufficient to significantly increase ODC activity. In contrast, supplementation with D-methionine, L-lysine, L-aspartate or L-glutamate had little or no effect on ODC activity, whereas supplemental L-methionine, L-arginine, L-ornithine or L-cysteine was inhibitory. Polyamine assays showed that the putrescine content of cells varied in accordance with the changes in ODC activity. Western-blot and Northern-blot analyses revealed specifically increased levels of ODC protein but not mRNA, respectively, in response to supplementation with an ODC-inducing amino acid. Suppression of the increase in cycloheximide-treated cells confirmed a requirement for protein synthesis. Pulse-labelling of cells with [S-35]methionine showed a 3-fold increase in the synthesis of ODC protein after 4 h of supplementation with glycine or L-serine. Supplemental glycine also augmented, reversibly, the half-life of ODC by almost 4-fold and simultaneously decreased the activity of putrescine-induced free antizyme. These results suggest that translational, but not transcriptional, regulation of ODC takes part in ODC induction by amino acids in Caco-2 cells, However, it also appears to occur in concert with decreased enzyme inactivation and/or degradation.
引用
收藏
页码:401 / 408
页数:8
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