Identification of a basolateral Cl-/HCO3- exchanger specific to gastric parietal cells

被引：80

作者：

Petrovic, S

Ju, X

Barone, S

Seidler, U

Alper, SL

Lohi, H

Kere, J

Soleimani, M

机构：

[1] Univ Cincinnati, Dept Med, Cincinnati, OH 45267 USA

[2] Vet Adm Med Ctr, Cincinnati, OH 45220 USA

[3] Univ Tubingen, Dept Med, D-72076 Tubingen, Germany

[4] Harvard Univ, Dept Med, Boston, MA 02215 USA

[5] Univ Helsinki, Dept Med Genet, Helsinki 00014, Finland

[6] Karolinska Inst, Dept Biosci Novum, S-14157 Huddinge, Sweden

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2003年 / 284卷 / 06期

关键词：

SLC26A7; AE2;

D O I：

10.1152/ajpgi.00454.2002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The basolateral Cl-/ HCO3- exchanger in parietal cells plays an essential role in gastric acid secretion mediated via the apical gastric H+-K+-ATPase. Here, we report the identification of a new Cl-/HCO3- exchanger, which shows exclusive expression in mouse stomach and kidney, with expression in the stomach limited to the basolateral membrane of gastric parietal cells. Tissue distribution studies by RT-PCR and Northern hybridizations demonstrated the exclusive expression of this transporter, also known as SLC26A7, to stomach and kidney, with the stomach expression significantly more abundant. No expression was detected in the intestine. Cellular distribution studies by RT-PCR and Northern hybridizations demonstrated predominant localization of SLC26A7 in gastric parietal cells. Immunofluorescence labeling localized this exchanger exclusively to the basolateral membrane of gastric parietal cells, and functional studies in oocytes indicated that SLC26A7 is a DIDS-sensitive Cl-/HCO3- exchanger that is active in both acidic and alkaline pH(i). On the basis of its unique expression pattern and function, we propose that SLC26A7 is a basolateral Cl-/HCO3- exchanger in gastric parietal cells and plays a major role in gastric acid secretion.

引用

页码：G1093 / G1103

页数：11

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