Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures

被引：54

作者：

Weber, A

Maier, RF

Hoffmann, U

Grips, M

Hoppenz, M

Aktas, AG

Heinemann, U

Obladen, M

Schuchmann, S

机构：

[1] Humboldt Univ, Charite, Dept Neonatol, D-13353 Berlin, Germany

[2] Humboldt Univ, Charite, Johannes Muller Inst Physiol, D-10117 Berlin, Germany

来源：

BRAIN RESEARCH | 2002年 / 958卷 / 02期

关键词：

erythropoietin; synaptic transmission; ischemia; neuroprotection; hippocampal slice culture;

D O I：

10.1016/S0006-8993(02)03604-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress, In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 muM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures. (C) 2002 Elsevier Science B.V. All rights reserved.

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收藏

页码：305 / 311

页数：7

相关论文

共 19 条

[1] Dopamine depresses polysynaptic inhibition in rat subicular neurons [J].

Behr, J ;

Gloveli, T ;

Schmitz, D ;

Heinemann, U .

BRAIN RESEARCH, 2000, 861 (01) :160-164

[2] Activation of the transcription factor NF-κB by the erythropoietin receptor -: Structural requirements and biological significance [J].

Bittorf, T ;

Büchse, T ;

Sasse, T ;

Jaster, R ;

Brock, J .

CELLULAR SIGNALLING, 2001, 13 (09) :673-681

[3] Induction of tolerance in rat cortical neurons: hypoxic preconditioning [J].

Bruer, U ;

Weih, MK ;

Isaev, NK ;

Meisel, A ;

Ruscher, K ;

Bergk, A ;

Trendelenburg, G ;

Wiegand, F ;

Victorov, IV ;

Dirnagl, U .

FEBS LETTERS, 1997, 414 (01) :117-121

[4] Erythropoietin-mediated neuroprotection involves cross-talk between Jak2 and NF-κB signalling cascades [J].

Digicaylioglu, M ;

Lipton, SA .

NATURE, 2001, 412 (6847) :641-647

[5] Dopamine inhibition of evoked IPSCs in rat prefrontal cortex [J].

Gonzalez-Islas, C ;

Hablitz, JJ .

JOURNAL OF NEUROPHYSIOLOGY, 2001, 86 (06) :2911-2918

[6] SYNAPTIC ACTIVATION OF NF-KAPPA-B BY GLUTAMATE IN CEREBELLAR GRANULE NEURONS IN-VITRO [J].

GUERRINI, L ;

BLASI, F ;

DENISDONINI, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9077-9081

[7] Immunohistochemical localization of erythropoietin and its receptor in the developing human brain [J].

Juul, SE ;

Yachnis, AT ;

Rojiani, AM ;

Christensen, RD .

PEDIATRIC AND DEVELOPMENTAL PATHOLOGY, 1999, 2 (02) :148-158

[8] Erythropoietin inhibits calcium-induced neurotransmitter release from clonal neuronal cells [J].

Kawakami, M ;

Iwasaki, S ;

Sato, K ;

Takahashi, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 279 (01) :293-297

[9] Erythropoietin receptor-mediated inhibition of exocytotic glutamate release confers neuroprotection during chemical ischemia [J].

Kawakami, M ;

Sekiguchi, M ;

Sato, K ;

Kozaki, S ;

Takahashi, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (42) :39469-39475

[10] Effects of erythropoietin on neuronal activity [J].

Koshimura, K ;

Murakami, Y ;

Sohmiya, M ;

Tanaka, J ;

Kato, Y .

JOURNAL OF NEUROCHEMISTRY, 1999, 72 (06) :2565-2572