Use of a gastric juice-based PCR assay to detect Helicobacter pylori infection in culture-negative patients

被引：36

作者：

Yoshida, H

Hirota, K

Shiratori, Y

Nihei, T

Amano, S

Yoshida, A

Kawamata, O

Omata, M

机构：

[1] Univ Tokyo, Fac Med, Dept Internal Med 2, Bunkyo Ku, Tokyo 113, Japan

[2] Mito Saiseikai Hosp, Dept Internal Med, Mito, Ibaraki 31141, Japan

[3] SRL Inc, Ctr Mol Biol & Cytogenet, Tokyo 19203, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 01期

关键词：

D O I：

10.1128/JCM.36.1.317-320.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A gastric juice-based PCR assay was compared,vith culture, microscopy, and a rapid urease test with specimens from 114 subjects. The PCR and conventional tests were positive for 76 and 62% of the subjects, respectively. The prevalence of gastroduodenal disease and seropositivity for anti-Helicobacter pylori immunoglobulin G were similarly high among conventional-test-positive and PCR-only-positive subjects compared to all-negative ones. The PCR assay is recommended to confirm the H. pylori status of culture-negative peptic-ulcer patients.

引用

收藏

页码：317 / 320

页数：4

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